Respiratory properties of blood in the harbor porpoise, Phocoena phocoena

TY - JOUR

T1 - Respiratory properties of blood in the harbor porpoise, Phocoena phocoena

AU - Soegaard, Lisette B

AU - Hansen, Marie N

AU - van Elk, Cornelis

AU - Brahm, Jesper

AU - Jensen, Frank Bo

PY - 2012/6

Y1 - 2012/6

N2 - Harbor porpoises are active divers that exchange O2 and CO 2 with the environment during a fast single breath upon surfacing. We investigated blood O2-transporting properties, buffer characteristics, Cl- transport via the erythrocyte anion exchanger (AE1), circulating nitric oxide metabolites and hemoglobin nitrite reduction in harbor porpoises with the aim to evaluate traits that are adaptive for diving behavior. Blood O2 affinity was higher in harbor porpoises than in similar sized terrestrial mammals, as supported by our parallel recordings of O2 equilibria in sheep and pig blood. Further, O2 affinity tended to increase with increasing body mass. A high O2 affinity favors O2 extraction from the lungs, but a normal Bohr effect (ΔlogP50/ΔpH=-0.46) gradually lowers O2 affinity during dives (where CO2 accumulates) to assist O2 off-loading to perfused tissues. The true plasma non-bicarbonate buffer value was moderately higher than in terrestrial mammals and increased upon deoxygenation. Plasma bicarbonate was also relatively high, contributing to increase the overall buffer capacity. The apparent Cl- permeability of harbor porpoise erythrocytes was similar to the human value at 37°C, showing absence of a comparative increase in the velocity of erythrocyte HCO3-/Cl-exchange to aid CO2 excretion. The Q10 for AE1-mediated Cl- transport in harbor porpoises was lower than in humans and seemed to match the Q10 for metabolism (Q 10≈2). Plasma nitrite, plasma nitrate and hemoglobin-mediated nitrite reduction were elevated compared with mammalian standards, suggesting that increased nitric oxide bioavailability and nitrite-derived nitric oxide could play important roles in diving physiology.

AB - Harbor porpoises are active divers that exchange O2 and CO 2 with the environment during a fast single breath upon surfacing. We investigated blood O2-transporting properties, buffer characteristics, Cl- transport via the erythrocyte anion exchanger (AE1), circulating nitric oxide metabolites and hemoglobin nitrite reduction in harbor porpoises with the aim to evaluate traits that are adaptive for diving behavior. Blood O2 affinity was higher in harbor porpoises than in similar sized terrestrial mammals, as supported by our parallel recordings of O2 equilibria in sheep and pig blood. Further, O2 affinity tended to increase with increasing body mass. A high O2 affinity favors O2 extraction from the lungs, but a normal Bohr effect (ΔlogP50/ΔpH=-0.46) gradually lowers O2 affinity during dives (where CO2 accumulates) to assist O2 off-loading to perfused tissues. The true plasma non-bicarbonate buffer value was moderately higher than in terrestrial mammals and increased upon deoxygenation. Plasma bicarbonate was also relatively high, contributing to increase the overall buffer capacity. The apparent Cl- permeability of harbor porpoise erythrocytes was similar to the human value at 37°C, showing absence of a comparative increase in the velocity of erythrocyte HCO3-/Cl-exchange to aid CO2 excretion. The Q10 for AE1-mediated Cl- transport in harbor porpoises was lower than in humans and seemed to match the Q10 for metabolism (Q 10≈2). Plasma nitrite, plasma nitrate and hemoglobin-mediated nitrite reduction were elevated compared with mammalian standards, suggesting that increased nitric oxide bioavailability and nitrite-derived nitric oxide could play important roles in diving physiology.

U2 - 10.1242/jeb.069872

DO - 10.1242/jeb.069872

M3 - Journal article

C2 - 22573773

SN - 0022-0949

VL - 215

SP - 1938

EP - 1943

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

IS - Pt 11

ER -