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Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture
Jurriaan Hölzenspies,
Gelo Victoriano Dela Cruz
,
Joshua M Brickman
Stem Cell and developmental Biology - early embryonic lineage specification
Laboratory
2
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Keyphrases
Antibody Staining
25%
Cell Population
100%
Differentiation Markers
25%
Dynamic Cells
100%
Embryonic Stem Cell Culture
100%
Extraembryonic Lineages
25%
Feeder-free
100%
Fluorescence-activated Cell Sorting
100%
Highly Dynamic
25%
Mouse Embryonic Stem Cells (mESCs)
100%
Pluripotency Markers
25%
Pluripotent Cells
25%
Primitive Endoderm
25%
Reporter Lines
25%
Self-renewal
25%
Specific Populations
25%
Stage-specific Embryonic antigen-1 (SSEA-1)
25%
Transcriptional Heterogeneity
25%
Trophoblast
25%
Immunology and Microbiology
Cell Population
100%
Embryonic Stem Cell
75%
Endoderm
25%
Fluorescence Activated Cell Sorting
100%
Lineage
50%
Mouse Embryonic Stem Cell
100%
Stem Cell Culture
100%
Trophoblast
25%