Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men

Thomas Morville; Mads Rosenkilde; Thor Munch-Andersen; Peter Riis Andersen; Katja Kjær Groenbæk; Signe Helbo; Marianne Kristensen; Andreas Vigelsø; Nick Mattsson; Hanne Kruuse Rasmusen; Amelia Guadalupe-Grau; Angela Fago; Christina Neigaard Hansen; Brigitte Twelkmeyer; Jesper Løvind Andersen; Flemming Dela; Jørn Wulff Helge

doi:10.1249/mss.0000000000001107

Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men

Thomas Morville, Mads Rosenkilde, Thor Munch-Andersen, Peter Riis Andersen, Katja Kjær Groenbæk, Signe Helbo, Marianne Kristensen, Andreas Vigelsø, Nick Mattsson, Hanne Kruuse Rasmusen, Amelia Guadalupe-Grau, Angela Fago, Christina Neigaard Hansen, Brigitte Twelkmeyer, Jesper Løvind Andersen, Flemming Dela, Jørn Wulff Helge

5 Citationer (Scopus)

Abstract

Introduction/Purpose Fat metabolism and muscle adaptation was investigated in six older trained men (age, 61 ± 4 yr; V-O_2max, 48 ± 2 mL·kg^-1·min^-1) after repeated prolonged exercise). Methods A distance of 2706 km (1681 miles) cycling was performed over 14 d, and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 h after the completion of the cycling. V-O_2max and maximal fat oxidation were measured using incremental exercise tests. HR was continuously sampled during cycling to estimate exercise intensity. Results The daily duration of exercise was 10 h and 31 ± 37 min, and the mean intensity was 53% ± 1% of V-O_2max. Body weight remained unchanged. V-O_2max and maximal fat oxidation rate decreased by 6% ± 2% (P = 0.04) and 32% ± 8% (P < 0.01), respectively. The exercise intensity that elicits maximal fat oxidation was not significantly decreased. Plasma free fatty acid (FA) concentration decreased (P < 0.002) from 500 ± 77 μmol·L^-1 to 160 ± 38 μmol·L^-1. Plasma glucose concentration as well as muscle glycogen, myoglobin, and triacylglycerol content remained unchanged. Muscle citrate synthase and ß-hydroxy-acyl-CoA-dehydrogenase activities were unchanged, but the protein expression of HKII, GLUT4, and adipose triacylglycerol lipase were significantly increased. Conclusions Overall, the decreased maximal fat oxidation was probably due to lower exogenous plasma fatty acid availability and the muscle adaptation pattern indicates an increased glucose transport capacity and an increased muscle lipolysis capacity supporting an increased contribution of exogenous glucose and endogenous fat during exercise.

Originalsprog	Engelsk
Tidsskrift	Medicine and Science in Sports and Exercise
Vol/bind	49
Udgave nummer	2
Sider (fra-til)	308-316
Antal sider	9
ISSN	0195-9131
DOI	https://doi.org/10.1249/mss.0000000000001107
Status	Udgivet - 1 feb. 2017

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10.1249/mss.0000000000001107

Citationsformater

Morville, T., Rosenkilde, M., Munch-Andersen, T., Riis Andersen, P., Groenbæk, K. K., Helbo, S., Kristensen, M., Vigelsø, A., Mattsson, N., Kruuse Rasmusen, H., Guadalupe-Grau, A., Fago, A., Neigaard Hansen, C., Twelkmeyer, B., Løvind Andersen, J., Dela, F., & Wulff Helge, J. (2017). Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men. Medicine and Science in Sports and Exercise, 49(2), 308-316. https://doi.org/10.1249/mss.0000000000001107

Morville, T, Rosenkilde, M, Munch-Andersen, T, Riis Andersen, P, Groenbæk, KK, Helbo, S, Kristensen, M, Vigelsø, A, Mattsson, N, Kruuse Rasmusen, H, Guadalupe-Grau, A, Fago, A, Neigaard Hansen, C, Twelkmeyer, B, Løvind Andersen, J, Dela, F & Wulff Helge, J 2017, 'Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men', Medicine and Science in Sports and Exercise, bind 49, nr. 2, s. 308-316. https://doi.org/10.1249/mss.0000000000001107

@article{f04161303cd8490c962045f3405ed6db,

title = "Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men",

abstract = "Introduction/Purpose Fat metabolism and muscle adaptation was investigated in six older trained men (age, 61 ± 4 yr; V-O2max, 48 ± 2 mL·kg-1·min-1) after repeated prolonged exercise). Methods A distance of 2706 km (1681 miles) cycling was performed over 14 d, and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 h after the completion of the cycling. V-O2max and maximal fat oxidation were measured using incremental exercise tests. HR was continuously sampled during cycling to estimate exercise intensity. Results The daily duration of exercise was 10 h and 31 ± 37 min, and the mean intensity was 53% ± 1% of V-O2max. Body weight remained unchanged. V-O2max and maximal fat oxidation rate decreased by 6% ± 2% (P = 0.04) and 32% ± 8% (P < 0.01), respectively. The exercise intensity that elicits maximal fat oxidation was not significantly decreased. Plasma free fatty acid (FA) concentration decreased (P < 0.002) from 500 ± 77 μmol·L-1 to 160 ± 38 μmol·L-1. Plasma glucose concentration as well as muscle glycogen, myoglobin, and triacylglycerol content remained unchanged. Muscle citrate synthase and {\ss}-hydroxy-acyl-CoA-dehydrogenase activities were unchanged, but the protein expression of HKII, GLUT4, and adipose triacylglycerol lipase were significantly increased. Conclusions Overall, the decreased maximal fat oxidation was probably due to lower exogenous plasma fatty acid availability and the muscle adaptation pattern indicates an increased glucose transport capacity and an increased muscle lipolysis capacity supporting an increased contribution of exogenous glucose and endogenous fat during exercise.",

author = "Thomas Morville and Mads Rosenkilde and Thor Munch-Andersen and {Riis Andersen}, Peter and Groenb{\ae}k, {Katja Kj{\ae}r} and Signe Helbo and Marianne Kristensen and Andreas Vigels{\o} and Nick Mattsson and {Kruuse Rasmusen}, Hanne and Amelia Guadalupe-Grau and Angela Fago and {Neigaard Hansen}, Christina and Brigitte Twelkmeyer and {L{\o}vind Andersen}, Jesper and Flemming Dela and {Wulff Helge}, J{\o}rn",

year = "2017",

month = feb,

day = "1",

doi = "10.1249/mss.0000000000001107",

language = "English",

volume = "49",

pages = "308--316",

journal = "Medicine and Science in Sports and Exercise",

issn = "0195-9131",

publisher = "Lippincott Williams & Wilkins",

number = "2",

}

TY - JOUR

T1 - Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men

AU - Morville, Thomas

AU - Rosenkilde, Mads

AU - Munch-Andersen, Thor

AU - Riis Andersen, Peter

AU - Groenbæk, Katja Kjær

AU - Helbo, Signe

AU - Kristensen, Marianne

AU - Vigelsø, Andreas

AU - Mattsson, Nick

AU - Kruuse Rasmusen, Hanne

AU - Guadalupe-Grau, Amelia

AU - Fago, Angela

AU - Neigaard Hansen, Christina

AU - Twelkmeyer, Brigitte

AU - Løvind Andersen, Jesper

AU - Dela, Flemming

AU - Wulff Helge, Jørn

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Introduction/Purpose Fat metabolism and muscle adaptation was investigated in six older trained men (age, 61 ± 4 yr; V-O2max, 48 ± 2 mL·kg-1·min-1) after repeated prolonged exercise). Methods A distance of 2706 km (1681 miles) cycling was performed over 14 d, and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 h after the completion of the cycling. V-O2max and maximal fat oxidation were measured using incremental exercise tests. HR was continuously sampled during cycling to estimate exercise intensity. Results The daily duration of exercise was 10 h and 31 ± 37 min, and the mean intensity was 53% ± 1% of V-O2max. Body weight remained unchanged. V-O2max and maximal fat oxidation rate decreased by 6% ± 2% (P = 0.04) and 32% ± 8% (P < 0.01), respectively. The exercise intensity that elicits maximal fat oxidation was not significantly decreased. Plasma free fatty acid (FA) concentration decreased (P < 0.002) from 500 ± 77 μmol·L-1 to 160 ± 38 μmol·L-1. Plasma glucose concentration as well as muscle glycogen, myoglobin, and triacylglycerol content remained unchanged. Muscle citrate synthase and ß-hydroxy-acyl-CoA-dehydrogenase activities were unchanged, but the protein expression of HKII, GLUT4, and adipose triacylglycerol lipase were significantly increased. Conclusions Overall, the decreased maximal fat oxidation was probably due to lower exogenous plasma fatty acid availability and the muscle adaptation pattern indicates an increased glucose transport capacity and an increased muscle lipolysis capacity supporting an increased contribution of exogenous glucose and endogenous fat during exercise.

AB - Introduction/Purpose Fat metabolism and muscle adaptation was investigated in six older trained men (age, 61 ± 4 yr; V-O2max, 48 ± 2 mL·kg-1·min-1) after repeated prolonged exercise). Methods A distance of 2706 km (1681 miles) cycling was performed over 14 d, and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 h after the completion of the cycling. V-O2max and maximal fat oxidation were measured using incremental exercise tests. HR was continuously sampled during cycling to estimate exercise intensity. Results The daily duration of exercise was 10 h and 31 ± 37 min, and the mean intensity was 53% ± 1% of V-O2max. Body weight remained unchanged. V-O2max and maximal fat oxidation rate decreased by 6% ± 2% (P = 0.04) and 32% ± 8% (P < 0.01), respectively. The exercise intensity that elicits maximal fat oxidation was not significantly decreased. Plasma free fatty acid (FA) concentration decreased (P < 0.002) from 500 ± 77 μmol·L-1 to 160 ± 38 μmol·L-1. Plasma glucose concentration as well as muscle glycogen, myoglobin, and triacylglycerol content remained unchanged. Muscle citrate synthase and ß-hydroxy-acyl-CoA-dehydrogenase activities were unchanged, but the protein expression of HKII, GLUT4, and adipose triacylglycerol lipase were significantly increased. Conclusions Overall, the decreased maximal fat oxidation was probably due to lower exogenous plasma fatty acid availability and the muscle adaptation pattern indicates an increased glucose transport capacity and an increased muscle lipolysis capacity supporting an increased contribution of exogenous glucose and endogenous fat during exercise.

U2 - 10.1249/mss.0000000000001107

DO - 10.1249/mss.0000000000001107

M3 - Journal article

C2 - 27685008

SN - 0195-9131

VL - 49

SP - 308

EP - 316

JO - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

IS - 2

ER -

Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men

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