Relation of intracellular signal levels and promoter activities in the gal regulon of Escherichia coli

TY - JOUR

T1 - Relation of intracellular signal levels and promoter activities in the gal regulon of Escherichia coli

AU - Krishna, Sandeep

AU - Orosz, László

AU - Sneppen, Kim

AU - Adhya, Sankar

AU - Semsey, Szabolcs

PY - 2009/8/28

Y1 - 2009/8/28

N2 - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.

AB - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Galactose

KW - Gene Expression Regulation, Bacterial

KW - Promoter Regions, Genetic

KW - Regulon

KW - Signal Transduction

KW - Transcription Factors

KW - Journal Article

KW - Research Support, N.I.H., Intramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jmb.2009.06.043

DO - 10.1016/j.jmb.2009.06.043

M3 - Journal article

C2 - 19559028

SN - 0022-2836

VL - 391

SP - 671

EP - 678

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -