TY - JOUR
T1 - Regeneration of irradiated salivary glands with stem cell marker expressing cells
AU - Nanduri, Lalitha S Y
AU - Maimets, Martti
AU - Pringle, Sarah A
AU - van der Zwaag, Marianne
AU - van Os, Ronald P
AU - Coppes, Robert P
N1 - Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
PY - 2011/6
Y1 - 2011/6
N2 - BACKGROUND: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.METHODS: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.RESULTS: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.CONCLUSIONS: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland.
AB - BACKGROUND: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.METHODS: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.RESULTS: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.CONCLUSIONS: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland.
KW - AC133 Antigen
KW - Animals
KW - Antigens, CD/metabolism
KW - CD24 Antigen/metabolism
KW - Female
KW - Flow Cytometry
KW - Glycoproteins/metabolism
KW - Head and Neck Neoplasms/radiotherapy
KW - Immunoenzyme Techniques
KW - Integrin alpha6/metabolism
KW - Mice
KW - Mice, Inbred C57BL
KW - Peptides/metabolism
KW - Proto-Oncogene Proteins c-kit/metabolism
KW - Regeneration
KW - Salivary Glands/cytology
KW - Stem Cell Transplantation/methods
KW - Stem Cells/cytology
KW - Xerostomia/etiology
U2 - 10.1016/j.radonc.2011.05.085
DO - 10.1016/j.radonc.2011.05.085
M3 - Journal article
C2 - 21719134
SN - 0167-8140
VL - 99
SP - 367
EP - 372
JO - Radiotherapy & Oncology
JF - Radiotherapy & Oncology
IS - 3
ER -