Reduction of Sb(V) in a human macrophage cell line measured by HPLC-ICP-MS

TY - JOUR

T1 - Reduction of Sb(V) in a human macrophage cell line measured by HPLC-ICP-MS

AU - Hansen, Claus

AU - Hansen, Erik Wind

AU - Hansen, Helle Rüsz

AU - Gammelgaard, Bente

AU - Stürup, Stefan

PY - 2011/12

Y1 - 2011/12

N2 - Drugs based on pentavalent antimony are first-line treatment of the parasite disease leishmaniasis. It is generally believed that Sb(V) acts as a prodrug, which is activated by reduction to Sb(III); however, the site of reduction is not known. It has been hypothesised that the reduction takes place in the parasites' host cells, the macrophages. In this study, the human macrophage cell line Mono Mac 6 was exposed to Sb(V) in form of the drug sodium stibogluconate (Pentostam™). Cell extracts were analysed for Sb species by high-performance liquid chromatography with inductively coupled plasma-mass spectrometry detection. We found that Sb(V) is actually reduced to Sb(III) in the macrophages; up to 23% of the intracellular Sb was found as Sb(III). Transfer of the cells to Sb-free medium rapidly decreased their Sb(V) and Sb(III) content. Induction of the cell's production of reactive oxygen species did not have any marked effect on the intracellular amounts of Sb(III).

AB - Drugs based on pentavalent antimony are first-line treatment of the parasite disease leishmaniasis. It is generally believed that Sb(V) acts as a prodrug, which is activated by reduction to Sb(III); however, the site of reduction is not known. It has been hypothesised that the reduction takes place in the parasites' host cells, the macrophages. In this study, the human macrophage cell line Mono Mac 6 was exposed to Sb(V) in form of the drug sodium stibogluconate (Pentostam™). Cell extracts were analysed for Sb species by high-performance liquid chromatography with inductively coupled plasma-mass spectrometry detection. We found that Sb(V) is actually reduced to Sb(III) in the macrophages; up to 23% of the intracellular Sb was found as Sb(III). Transfer of the cells to Sb-free medium rapidly decreased their Sb(V) and Sb(III) content. Induction of the cell's production of reactive oxygen species did not have any marked effect on the intracellular amounts of Sb(III).

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/s12011-011-9079-9

DO - 10.1007/s12011-011-9079-9

M3 - Journal article

SN - 0163-4984

VL - 144

SP - 234

EP - 243

JO - Biological Trace Element Research

JF - Biological Trace Element Research

IS - 1-3

ER -