Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

TY - JOUR

T1 - Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

AU - Nour-Eldin, Hussam Hassan

AU - Madsen, Svend Roesen

AU - Engelen, Steven

AU - Jørgensen, Morten Egevang

AU - Olsen, Carl Erik

AU - Andersen, Jonathan Sonne

AU - Seynnaeve, David

AU - Verhoye, Thalia

AU - Fulawka, Rudy

AU - Denolf, Peter

AU - Halkier, Barbara Ann

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.

AB - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.

KW - Brassica

KW - Genetic Enhancement

KW - Glucosinolates

KW - Monosaccharide Transport Proteins

KW - Mutation

KW - Plant Oils

KW - Plants, Genetically Modified

KW - Seeds

KW - Journal Article

U2 - 10.1038/nbt.3823

DO - 10.1038/nbt.3823

M3 - Journal article

C2 - 28288105

SN - 1087-0156

VL - 35

SP - 377

EP - 382

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 4

ER -