Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis

Yuzuki Manabe; Yves Verhertbruggen; Sascha Gille; Jesper Harholt; Sun-Li Chong; Prashant Mohan-Anupama Pawar; Ewa J. Mellerowicz; Maija Tenkanen; Kun Cheng; Markus Pauly; Henrik Vibe Scheller

doi:10.1104/pp.113.225193

Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis

Yuzuki Manabe, Yves Verhertbruggen, Sascha Gille, Jesper Harholt, Sun-Li Chong, Prashant Mohan-Anupama Pawar, Ewa J. Mellerowicz, Maija Tenkanen, Kun Cheng, Markus Pauly, Henrik Vibe Scheller

51 Citationer (Scopus)

Abstract

The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco)mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.

Originalsprog	Engelsk
Tidsskrift	Plant Physiology
Vol/bind	163
Udgave nummer	3
Sider (fra-til)	1107-1117
Antal sider	11
ISSN	0032-0889
DOI	https://doi.org/10.1104/pp.113.225193
Status	Udgivet - 1 nov. 2013

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10.1104/pp.113.225193

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title = "Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis",

abstract = "The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco)mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.",

author = "Yuzuki Manabe and Yves Verhertbruggen and Sascha Gille and Jesper Harholt and Sun-Li Chong and Pawar, {Prashant Mohan-Anupama} and Mellerowicz, {Ewa J.} and Maija Tenkanen and Kun Cheng and Markus Pauly and Scheller, {Henrik Vibe}",

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doi = "10.1104/pp.113.225193",

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T1 - Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis

AU - Manabe, Yuzuki

AU - Verhertbruggen, Yves

AU - Gille, Sascha

AU - Harholt, Jesper

AU - Chong, Sun-Li

AU - Pawar, Prashant Mohan-Anupama

AU - Mellerowicz, Ewa J.

AU - Tenkanen, Maija

AU - Cheng, Kun

AU - Pauly, Markus

AU - Scheller, Henrik Vibe

PY - 2013/11/1

Y1 - 2013/11/1

N2 - The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco)mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.

AB - The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco)mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.

U2 - 10.1104/pp.113.225193

DO - 10.1104/pp.113.225193

M3 - Journal article

C2 - 24019426

SN - 0032-0889

VL - 163

SP - 1107

EP - 1117

JO - Plant Physiology

JF - Plant Physiology

IS - 3

ER -

Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis

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