Recapitulating and Deciphering Human Pancreas Development From Human Pluripotent Stem Cells in a Dish

TY - CHAP

T1 - Recapitulating and Deciphering Human Pancreas Development From Human Pluripotent Stem Cells in a Dish

AU - Petersen, Maja B K

AU - Gonçalves, Carla A C

AU - Kim, Yung Hae

AU - Grapin-Botton, Anne

N1 - © 2018 Elsevier Inc. All rights reserved.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Here, we review how human pluripotent stem cell models of pancreas development have emerged and became an important tool to study human development and disease. Initially developed toward the production of β cells for diabetes therapy, the protocols have been refined based on knowledge of pancreas development in model organisms. While the cells produced are closer and closer to the end goal of a functional β cell, these models have also been used to carry out functional experiments addressing gene function and expression as well as regulatory and epigenetic landscape changes during human pancreas development. They thereby complement model organisms and reports from human genetic variants predisposing to different forms of diabetes, as well as observations on human fetal tissue. In this review, we therefore compare these different sources of information and discuss how human stem cell models are evolving to inform us on pancreatic diseases and possible treatments.

AB - Here, we review how human pluripotent stem cell models of pancreas development have emerged and became an important tool to study human development and disease. Initially developed toward the production of β cells for diabetes therapy, the protocols have been refined based on knowledge of pancreas development in model organisms. While the cells produced are closer and closer to the end goal of a functional β cell, these models have also been used to carry out functional experiments addressing gene function and expression as well as regulatory and epigenetic landscape changes during human pancreas development. They thereby complement model organisms and reports from human genetic variants predisposing to different forms of diabetes, as well as observations on human fetal tissue. In this review, we therefore compare these different sources of information and discuss how human stem cell models are evolving to inform us on pancreatic diseases and possible treatments.

U2 - 10.1016/bs.ctdb.2018.02.009

DO - 10.1016/bs.ctdb.2018.02.009

M3 - Book chapter

C2 - 29801529

SN - 978-0-12-804251-9

VL - 129

T3 - Current Topics in Developmental Biology

SP - 143

EP - 190

BT - Human Embryonic Stem Cells in Development

PB - Elsevier

ER -