Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique

Thomas T Thomsen; Laura B Madsen; Helle H Hansson; Elsa V E Tomás; Derek Charlwood; Ib C Bygbjerg; Michael Alifrangis

doi:10.4269/ajtmh.12-0525

Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique

Thomas T Thomsen, Laura B Madsen, Helle H Hansson, Elsa V E Tomás, Derek Charlwood, Ib C Bygbjerg, Michael Alifrangis

33 Citationer (Scopus)

Abstract

Chloroquine (CQ) use in Mozambique was stopped in 2002 and artemether-lumefantrine (AL) was implemented in 2008. In light of no use of CQ and extensive use of AL, we determined the frequency of molecular markers of Plasmodium falciparum drug resistance/tolerance to CQ and AL in persons living in Linga-Linga, an isolated peninsula and in Furvela village, which is located 8 km inland. The P. falciparum chloroquine resistance transporter gene CVMNK wild type increased in frequency from 43.9% in 2009 to 66.4% in 2010 (P = 0.001), and combined P. falciparum multidrug resistance gene 1 N86-184F-D1246 haplotype increased significantly between years (P = 0.039). The combination of P. falciparum chloroquine resistance transporter gene CVMNK and P. falciparum multidrug resistance gene NFD increased from 24.3% (2009) to 45.3% in (2010, P = 0.017). The rapid changes observed may largely be caused by decreased use of CQ and large-scale use of AL. In the absence of a clear AL-resistance marker and the (almost) continent-wide use of AL in sub-Saharan Africa, and when considering CQ reintroduction, continued monitoring of these markers is needed.

Originalsprog	Engelsk
Tidsskrift	American Journal of Tropical Medicine and Hygiene
Vol/bind	88
Udgave nummer	3
Sider (fra-til)	536-41
Antal sider	6
ISSN	0002-9637
DOI	https://doi.org/10.4269/ajtmh.12-0525
Status	Udgivet - mar. 2013

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10.4269/ajtmh.12-0525

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Citationsformater

Thomsen, T. T., Madsen, L. B., Hansson, H. H., Tomás, E. V. E., Charlwood, D., Bygbjerg, I. C., & Alifrangis, M. (2013). Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique. American Journal of Tropical Medicine and Hygiene, 88(3), 536-41. https://doi.org/10.4269/ajtmh.12-0525

Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique. / Thomsen, Thomas T; Madsen, Laura B; Hansson, Helle H et al.

I: American Journal of Tropical Medicine and Hygiene, Bind 88, Nr. 3, 03.2013, s. 536-41.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Thomsen, TT, Madsen, LB, Hansson, HH, Tomás, EVE, Charlwood, D, Bygbjerg, IC & Alifrangis, M 2013, 'Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique', American Journal of Tropical Medicine and Hygiene, bind 88, nr. 3, s. 536-41. https://doi.org/10.4269/ajtmh.12-0525

Thomsen TT, Madsen LB, Hansson HH, Tomás EVE, Charlwood D, Bygbjerg IC et al. Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique. American Journal of Tropical Medicine and Hygiene. 2013 mar.;88(3):536-41. doi: 10.4269/ajtmh.12-0525

Thomsen, Thomas T ; Madsen, Laura B ; Hansson, Helle H et al. / Rapid selection of Plasmodium falciparum chloroquine resistance transporter gene and multidrug resistance gene-1 haplotypes associated with past chloroquine and present artemether-lumefantrine use in Inhambane District, southern Mozambique. I: American Journal of Tropical Medicine and Hygiene. 2013 ; Bind 88, Nr. 3. s. 536-41.

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abstract = "Chloroquine (CQ) use in Mozambique was stopped in 2002 and artemether-lumefantrine (AL) was implemented in 2008. In light of no use of CQ and extensive use of AL, we determined the frequency of molecular markers of Plasmodium falciparum drug resistance/tolerance to CQ and AL in persons living in Linga-Linga, an isolated peninsula and in Furvela village, which is located 8 km inland. The P. falciparum chloroquine resistance transporter gene CVMNK wild type increased in frequency from 43.9% in 2009 to 66.4% in 2010 (P = 0.001), and combined P. falciparum multidrug resistance gene 1 N86-184F-D1246 haplotype increased significantly between years (P = 0.039). The combination of P. falciparum chloroquine resistance transporter gene CVMNK and P. falciparum multidrug resistance gene NFD increased from 24.3% (2009) to 45.3% in (2010, P = 0.017). The rapid changes observed may largely be caused by decreased use of CQ and large-scale use of AL. In the absence of a clear AL-resistance marker and the (almost) continent-wide use of AL in sub-Saharan Africa, and when considering CQ reintroduction, continued monitoring of these markers is needed.",

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AU - Madsen, Laura B

AU - Hansson, Helle H

AU - Tomás, Elsa V E

AU - Charlwood, Derek

AU - Bygbjerg, Ib C

AU - Alifrangis, Michael

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AB - Chloroquine (CQ) use in Mozambique was stopped in 2002 and artemether-lumefantrine (AL) was implemented in 2008. In light of no use of CQ and extensive use of AL, we determined the frequency of molecular markers of Plasmodium falciparum drug resistance/tolerance to CQ and AL in persons living in Linga-Linga, an isolated peninsula and in Furvela village, which is located 8 km inland. The P. falciparum chloroquine resistance transporter gene CVMNK wild type increased in frequency from 43.9% in 2009 to 66.4% in 2010 (P = 0.001), and combined P. falciparum multidrug resistance gene 1 N86-184F-D1246 haplotype increased significantly between years (P = 0.039). The combination of P. falciparum chloroquine resistance transporter gene CVMNK and P. falciparum multidrug resistance gene NFD increased from 24.3% (2009) to 45.3% in (2010, P = 0.017). The rapid changes observed may largely be caused by decreased use of CQ and large-scale use of AL. In the absence of a clear AL-resistance marker and the (almost) continent-wide use of AL in sub-Saharan Africa, and when considering CQ reintroduction, continued monitoring of these markers is needed.

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