Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Erik Christian Løvbjerg Trampe; J. Kolbowski; U. Schreiber; M. Kuhl

doi:10.1007/s00227-011-1663-1

Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Erik Christian Løvbjerg Trampe, J. Kolbowski, U. Schreiber, M. Kuhl

Marine Biological Section

33 Citationer (Scopus)

Abstract

We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples

Originalsprog	Engelsk
Tidsskrift	Marine Biology
Vol/bind	158
Udgave nummer	7
Sider (fra-til)	1667-1675
Antal sider	9
ISSN	0025-3162
DOI	https://doi.org/10.1007/s00227-011-1663-1
Status	Udgivet - jul. 2011

Emneord

FAST REPETITION RATE
INDIVIDUAL ALGAL CELLS
A FLUORESCENCE
IN-VIVO
MICROBIAL COMMUNITIES
MARINE-PHYTOPLANKTON
LIGHT CURVES
PERFORMANCE
FLUOROMETRY
SEA

Adgang til dokumentet

10.1007/s00227-011-1663-1

Citationsformater

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abstract = "We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples",

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AU - Kolbowski, J.

AU - Schreiber, U.

AU - Kuhl, M.

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AB - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples

KW - FAST REPETITION RATE

KW - INDIVIDUAL ALGAL CELLS

KW - A FLUORESCENCE

KW - IN-VIVO

KW - MICROBIAL COMMUNITIES

KW - MARINE-PHYTOPLANKTON

KW - LIGHT CURVES

KW - PERFORMANCE

KW - FLUOROMETRY

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