Rapid assessment of different oxygenic phototrophs and single cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Erik Trampe; J. Kolbowski; U. Schreiber; Michael Kühl

Rapid assessment of different oxygenic phototrophs and single cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Erik Trampe, J. Kolbowski, U. Schreiber, Michael Kühl

Marine Biological Section

33 Citationer (Scopus)

Abstract

We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i. e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.

Originalsprog	Engelsk
Publikationsdato	jul. 2011
Status	Udgivet - jul. 2011
Begivenhed	BioImaging Workshop 2011 - Copenhagen, Danmark Varighed: 22 jun. 2011 → 23 jul. 2011

Konference

Konference	BioImaging Workshop 2011
Land/Område	Danmark
By	Copenhagen
Periode	22/06/2011 → 23/07/2011

Citationsformater

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title = "Rapid assessment of different oxygenic phototrophs and single cell photosynthesis with multicolour variable chlorophyll fluorescence imaging",

abstract = "We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i. e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.",

author = "Erik Trampe and J. Kolbowski and U. Schreiber and Michael K{\"u}hl",

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T1 - Rapid assessment of different oxygenic phototrophs and single cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

AU - Trampe, Erik

AU - Kolbowski, J.

AU - Schreiber, U.

AU - Kühl, Michael

PY - 2011/7

Y1 - 2011/7

N2 - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i. e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.

AB - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i. e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.

M3 - Poster

T2 - BioImaging Workshop 2011

Y2 - 22 June 2011 through 23 July 2011

ER -

Rapid assessment of different oxygenic phototrophs and single cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Abstract

Konference

Fingeraftryk

Citationsformater