TY - JOUR
T1 - Quantitative miRNA expression analysis: comparing microarrays with next-generation sequencing
AU - Willenbrock, Hanni
AU - Salomon, Jesper
AU - Søkilde, Rolf
AU - Barken, Kim Bundvig
AU - Hansen, Thomas Nøhr
AU - Nielsen, Finn Cilius
AU - Møller, Søren
AU - Litman, Thomas
N1 - Keywords: Gene Expression; MicroRNAs; Oligonucleotide Array Sequence Analysis; Reproducibility of Results; Sequence Analysis, RNA
PY - 2009
Y1 - 2009
N2 - Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
AB - Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
U2 - 10.1261/rna.1699809
DO - 10.1261/rna.1699809
M3 - Journal article
C2 - 19745027
SN - 1355-8382
VL - 15
SP - 2028
EP - 2034
JO - RNA: A publication of the RNA Society
JF - RNA: A publication of the RNA Society
IS - 11
ER -