Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

Claus Hansen; Bjørn Schmidt; Erik Huusfeldt Larsen; Bente Gammelgaard; Stefan Stürup; Helle Rüsz Hansen

doi:10.1039/c0an00796j

Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

Claus Hansen, Bjørn Schmidt, Erik Huusfeldt Larsen, Bente Gammelgaard, Stefan Stürup, Helle Rüsz Hansen

12 Citationer (Scopus)

Abstract

In solution antimony exists either in the pentavalent or trivalent oxidation state. As Sb(iii) is more toxic than Sb(v), it is important to be able to perform a quantitative speciation analysis of Sb's oxidation state. The most commonly applied chromatographic methods used for this redox speciation analysis do, however, often show a low chromatographic Sb recovery when samples of environmental or biological origin are analysed. In this study we explored basal chemistry of antimony and found that formation of macromolecules, presumably oligomeric and polymeric Sb(v) species, is the primary cause of low chromatographic recoveries. A combination of HPLC-ICP-MS, AFFF-ICP-MS and spin-filtration was applied for analysis of model compounds and biological samples. Quantitative chromatographic Sb redox speciation analysis was possible by acidic hydrolysis of the antimony polymers prior to analysis. Sample treatment procedures were studied and the optimum solution was acidic hydrolysis by 1 M HCl in the presence of chelating ligands (EDTA, citrate), which stabilise the trivalent oxidation state of Sb.

Originalsprog	Engelsk
Tidsskrift	Analyst
Vol/bind	136
Udgave nummer	5
Sider (fra-til)	996-1002
ISSN	0003-2654
DOI	https://doi.org/10.1039/c0an00796j
Status	Udgivet - 7 mar. 2011

Emneord

Det tidligere Farmaceutiske Fakultet

Adgang til dokumentet

10.1039/c0an00796j

Citationsformater

Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries. / Hansen, Claus; Schmidt, Bjørn; Larsen, Erik Huusfeldt et al.

I: Analyst, Bind 136, Nr. 5, 07.03.2011, s. 996-1002.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{dc46ca514d4949c19d847d056f75e045,

title = "Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries",

abstract = "In solution antimony exists either in the pentavalent or trivalent oxidation state. As Sb(iii) is more toxic than Sb(v), it is important to be able to perform a quantitative speciation analysis of Sb's oxidation state. The most commonly applied chromatographic methods used for this redox speciation analysis do, however, often show a low chromatographic Sb recovery when samples of environmental or biological origin are analysed. In this study we explored basal chemistry of antimony and found that formation of macromolecules, presumably oligomeric and polymeric Sb(v) species, is the primary cause of low chromatographic recoveries. A combination of HPLC-ICP-MS, AFFF-ICP-MS and spin-filtration was applied for analysis of model compounds and biological samples. Quantitative chromatographic Sb redox speciation analysis was possible by acidic hydrolysis of the antimony polymers prior to analysis. Sample treatment procedures were studied and the optimum solution was acidic hydrolysis by 1 M HCl in the presence of chelating ligands (EDTA, citrate), which stabilise the trivalent oxidation state of Sb.",

keywords = "Former Faculty of Pharmaceutical Sciences",

author = "Claus Hansen and Bj{\o}rn Schmidt and Larsen, {Erik Huusfeldt} and Bente Gammelgaard and Stefan St{\"u}rup and Hansen, {Helle R{\"u}sz}",

year = "2011",

month = mar,

day = "7",

doi = "10.1039/c0an00796j",

language = "English",

volume = "136",

pages = "996--1002",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "5",

}

TY - JOUR

T1 - Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

AU - Hansen, Claus

AU - Schmidt, Bjørn

AU - Larsen, Erik Huusfeldt

AU - Gammelgaard, Bente

AU - Stürup, Stefan

AU - Hansen, Helle Rüsz

PY - 2011/3/7

Y1 - 2011/3/7

N2 - In solution antimony exists either in the pentavalent or trivalent oxidation state. As Sb(iii) is more toxic than Sb(v), it is important to be able to perform a quantitative speciation analysis of Sb's oxidation state. The most commonly applied chromatographic methods used for this redox speciation analysis do, however, often show a low chromatographic Sb recovery when samples of environmental or biological origin are analysed. In this study we explored basal chemistry of antimony and found that formation of macromolecules, presumably oligomeric and polymeric Sb(v) species, is the primary cause of low chromatographic recoveries. A combination of HPLC-ICP-MS, AFFF-ICP-MS and spin-filtration was applied for analysis of model compounds and biological samples. Quantitative chromatographic Sb redox speciation analysis was possible by acidic hydrolysis of the antimony polymers prior to analysis. Sample treatment procedures were studied and the optimum solution was acidic hydrolysis by 1 M HCl in the presence of chelating ligands (EDTA, citrate), which stabilise the trivalent oxidation state of Sb.

AB - In solution antimony exists either in the pentavalent or trivalent oxidation state. As Sb(iii) is more toxic than Sb(v), it is important to be able to perform a quantitative speciation analysis of Sb's oxidation state. The most commonly applied chromatographic methods used for this redox speciation analysis do, however, often show a low chromatographic Sb recovery when samples of environmental or biological origin are analysed. In this study we explored basal chemistry of antimony and found that formation of macromolecules, presumably oligomeric and polymeric Sb(v) species, is the primary cause of low chromatographic recoveries. A combination of HPLC-ICP-MS, AFFF-ICP-MS and spin-filtration was applied for analysis of model compounds and biological samples. Quantitative chromatographic Sb redox speciation analysis was possible by acidic hydrolysis of the antimony polymers prior to analysis. Sample treatment procedures were studied and the optimum solution was acidic hydrolysis by 1 M HCl in the presence of chelating ligands (EDTA, citrate), which stabilise the trivalent oxidation state of Sb.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1039/c0an00796j

DO - 10.1039/c0an00796j

M3 - Journal article

C2 - 21157586

SN - 0003-2654

VL - 136

SP - 996

EP - 1002

JO - The Analyst

JF - The Analyst

IS - 5

ER -

Quantitative HPLC-ICP-MC analysis of antimony redox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

Abstract

Emneord

Adgang til dokumentet

Fingeraftryk

Citationsformater