Quantitation of Dam methyltransferase in Escherichia coli

E Boye; M G Marinus; A Løbner-Olesen

Quantitation of Dam methyltransferase in Escherichia coli

Functional genomics

83 Citationer (Scopus)

Abstract

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

Originalsprog	Engelsk
Tidsskrift	Journal of Bacteriology
Vol/bind	174
Udgave nummer	5
Sider (fra-til)	1682-5
Antal sider	4
ISSN	0021-9193
Status	Udgivet - mar. 1992

Citationsformater

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title = "Quantitation of Dam methyltransferase in Escherichia coli",

abstract = "An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.",

keywords = "Blotting, Western, Escherichia coli/enzymology, Escherichia coli Proteins, Immunoenzyme Techniques, Methyltransferases/analysis, Site-Specific DNA-Methyltransferase (Adenine-Specific)",

author = "E Boye and Marinus, {M G} and A L{\o}bner-Olesen",

year = "1992",

month = mar,

language = "English",

volume = "174",

pages = "1682--5",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "5",

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TY - JOUR

T1 - Quantitation of Dam methyltransferase in Escherichia coli

AU - Boye, E

AU - Marinus, M G

AU - Løbner-Olesen, A

PY - 1992/3

Y1 - 1992/3

N2 - An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

AB - An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

KW - Blotting, Western

KW - Escherichia coli/enzymology

KW - Escherichia coli Proteins

KW - Immunoenzyme Techniques

KW - Methyltransferases/analysis

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)

M3 - Journal article

C2 - 1537808

SN - 0021-9193

VL - 174

SP - 1682

EP - 1685

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 5

ER -

Quantitation of Dam methyltransferase in Escherichia coli

Abstract

Fingeraftryk

Citationsformater