Purinergic receptors stimulate Na+/Ca2+ exchange in pancreatic duct cells: possible role of proteins handling and transporting Ca2+

TY - JOUR

T1 - Purinergic receptors stimulate Na+/Ca2+ exchange in pancreatic duct cells: possible role of proteins handling and transporting Ca2+

AU - Hansen, Mette R

AU - Krabbe, Simon

AU - Ankorina-Stark, Ieva

AU - Novak, Ivana

N1 - Keywords: Animals; Calcium; Calcium-Binding Protein, Vitamin D-Dependent; Cell Line, Tumor; Epithelial Cells; Humans; Ion Transport; Pancreatic Ducts; Rats; Receptors, Calcium-Sensing; Receptors, Purinergic P2; Signal Transduction; Sodium-Calcium Exchanger

PY - 2009

Y1 - 2009

N2 - Most purinergic receptors activate intracellular Ca(2+) signalling, and in epithelia they stimulate transport of major ions. Aim of the present study on pancreatic ducts was to find whether P2 receptors also regulate cellular Ca(2+) transport, such as that via the Na(+)/Ca(2+) exchanger (NCX). Since NCX can also be connected with epithelial Ca(2+) transport, we also investigated expression of some Ca(2+)-handling/transporting proteins. Expression analysis revealed that pancreatic ducts of rat and human duct cell line CFPAC-1 (also PANC-1 and Capan-1) express the Na(+)/Ca(2+) exchanger (splice variants NCX1.3 and 1.7), as well as the Ca(2+)-sensing receptor and the Ca(2+)-binding protein calbindin-D 9k. These proteins localize to the luminal and lateral membranes of pancreatic duct epithelia. Ca(2+) imaging experiments showed that activation of purinergic P2Y and P2X receptors stimulated NCX in human and rat duct cells. Application of ATP to CFPAC-1 monolayers also stimulated Ca(2+) transport from the luminal to the basolateral side. Taken together, these results show that pancreatic ducts express a number of Ca(2+)-handling/transporting proteins and we propose that these together with purinergic receptors play a role in regulation of duct lumen Ca(2+) content.

AB - Most purinergic receptors activate intracellular Ca(2+) signalling, and in epithelia they stimulate transport of major ions. Aim of the present study on pancreatic ducts was to find whether P2 receptors also regulate cellular Ca(2+) transport, such as that via the Na(+)/Ca(2+) exchanger (NCX). Since NCX can also be connected with epithelial Ca(2+) transport, we also investigated expression of some Ca(2+)-handling/transporting proteins. Expression analysis revealed that pancreatic ducts of rat and human duct cell line CFPAC-1 (also PANC-1 and Capan-1) express the Na(+)/Ca(2+) exchanger (splice variants NCX1.3 and 1.7), as well as the Ca(2+)-sensing receptor and the Ca(2+)-binding protein calbindin-D 9k. These proteins localize to the luminal and lateral membranes of pancreatic duct epithelia. Ca(2+) imaging experiments showed that activation of purinergic P2Y and P2X receptors stimulated NCX in human and rat duct cells. Application of ATP to CFPAC-1 monolayers also stimulated Ca(2+) transport from the luminal to the basolateral side. Taken together, these results show that pancreatic ducts express a number of Ca(2+)-handling/transporting proteins and we propose that these together with purinergic receptors play a role in regulation of duct lumen Ca(2+) content.

U2 - 10.1159/000218185

DO - 10.1159/000218185

M3 - Journal article

C2 - 19471106

SN - 1015-8987

VL - 23

SP - 387

EP - 396

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

IS - 4-6

ER -