Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources

Katrine Togsverd-Bo; Luise W Idorn; Peter A Philipsen; Hans Christian Wulf; Merete Hædersdal

doi:10.1111/j.1600-0625.2012.01557.x

Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources

Katrine Togsverd-Bo, Luise W Idorn, Peter A Philipsen, Hans Christian Wulf, Merete Hædersdal

23 Citationer (Scopus)

Abstract

Topical photodynamic therapy (PDT) is used for various skin disorders,and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm²), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm²) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm²) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P < 0.001). In mid and deep dermis, fluorescence intensities were higher (37%) in HAL-treated skin than MAL-treated skin, although not significant (P = ns). At skin surface, photobleaching was significantly higher (90-98%) after LED illumination (18 and 37 J/cm²) than IPL (29-53%, 36 and 72 J/cm²) and LPDL (43-62%, 7 and 15 J/cm²) (P < 0.001). Within the skin, photobleaching was steady from epidermis to deep dermis by LED illumination (37 J/cm², P = ns), but declined from epidermis to mid and deep dermis for IPL-treated skinand LPDL-treated skin (IPL 72 J/cm²: 26-15%; LPDL 15 J/cm²: 37-23%) (P < 0.04). Clinically, erythema correlated linearly withMAL and HAL-induced photobleaching (r² = 0.175, P < 0.001). In conclusion, selective PpIX accumulation indicates HAL as an alternative to MAL for epidermal-targeted PDT. In clinically relevant doses, PpIX photobleaching throughout the skin was more profound following LED than LPDL and IPL exposure.

Originalsprog	Engelsk
Tidsskrift	Experimental Dermatology Online
Vol/bind	21
Udgave nummer	10
Sider (fra-til)	745-750
Antal sider	6
ISSN	1600-0625
DOI	https://doi.org/10.1111/j.1600-0625.2012.01557.x
Status	Udgivet - 2012

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10.1111/j.1600-0625.2012.01557.x

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Protoporphyrin IX formation and photobleaching in different layers of normal human skin : methyl- and hexylaminolevulinate and different light sources. / Togsverd-Bo, Katrine; Idorn, Luise W; Philipsen, Peter A et al.

I: Experimental Dermatology Online, Bind 21, Nr. 10, 2012, s. 745-750.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources",

abstract = "Topical photodynamic therapy (PDT) is used for various skin disorders,and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm2), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm2) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm2) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P < 0.001). In mid and deep dermis, fluorescence intensities were higher (37%) in HAL-treated skin than MAL-treated skin, although not significant (P = ns). At skin surface, photobleaching was significantly higher (90-98%) after LED illumination (18 and 37 J/cm2) than IPL (29-53%, 36 and 72 J/cm2) and LPDL (43-62%, 7 and 15 J/cm2) (P < 0.001). Within the skin, photobleaching was steady from epidermis to deep dermis by LED illumination (37 J/cm2, P = ns), but declined from epidermis to mid and deep dermis for IPL-treated skinand LPDL-treated skin (IPL 72 J/cm2: 26-15%; LPDL 15 J/cm2: 37-23%) (P < 0.04). Clinically, erythema correlated linearly withMAL and HAL-induced photobleaching (r2 = 0.175, P < 0.001). In conclusion, selective PpIX accumulation indicates HAL as an alternative to MAL for epidermal-targeted PDT. In clinically relevant doses, PpIX photobleaching throughout the skin was more profound following LED than LPDL and IPL exposure.",

author = "Katrine Togsverd-Bo and Idorn, {Luise W} and Philipsen, {Peter A} and Wulf, {Hans Christian} and Merete H{\ae}dersdal",

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T1 - Protoporphyrin IX formation and photobleaching in different layers of normal human skin

T2 - methyl- and hexylaminolevulinate and different light sources

AU - Togsverd-Bo, Katrine

AU - Idorn, Luise W

AU - Philipsen, Peter A

AU - Wulf, Hans Christian

AU - Hædersdal, Merete

PY - 2012

Y1 - 2012

N2 - Topical photodynamic therapy (PDT) is used for various skin disorders,and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm2), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm2) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm2) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P < 0.001). In mid and deep dermis, fluorescence intensities were higher (37%) in HAL-treated skin than MAL-treated skin, although not significant (P = ns). At skin surface, photobleaching was significantly higher (90-98%) after LED illumination (18 and 37 J/cm2) than IPL (29-53%, 36 and 72 J/cm2) and LPDL (43-62%, 7 and 15 J/cm2) (P < 0.001). Within the skin, photobleaching was steady from epidermis to deep dermis by LED illumination (37 J/cm2, P = ns), but declined from epidermis to mid and deep dermis for IPL-treated skinand LPDL-treated skin (IPL 72 J/cm2: 26-15%; LPDL 15 J/cm2: 37-23%) (P < 0.04). Clinically, erythema correlated linearly withMAL and HAL-induced photobleaching (r2 = 0.175, P < 0.001). In conclusion, selective PpIX accumulation indicates HAL as an alternative to MAL for epidermal-targeted PDT. In clinically relevant doses, PpIX photobleaching throughout the skin was more profound following LED than LPDL and IPL exposure.

AB - Topical photodynamic therapy (PDT) is used for various skin disorders,and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm2), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm2) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm2) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P < 0.001). In mid and deep dermis, fluorescence intensities were higher (37%) in HAL-treated skin than MAL-treated skin, although not significant (P = ns). At skin surface, photobleaching was significantly higher (90-98%) after LED illumination (18 and 37 J/cm2) than IPL (29-53%, 36 and 72 J/cm2) and LPDL (43-62%, 7 and 15 J/cm2) (P < 0.001). Within the skin, photobleaching was steady from epidermis to deep dermis by LED illumination (37 J/cm2, P = ns), but declined from epidermis to mid and deep dermis for IPL-treated skinand LPDL-treated skin (IPL 72 J/cm2: 26-15%; LPDL 15 J/cm2: 37-23%) (P < 0.04). Clinically, erythema correlated linearly withMAL and HAL-induced photobleaching (r2 = 0.175, P < 0.001). In conclusion, selective PpIX accumulation indicates HAL as an alternative to MAL for epidermal-targeted PDT. In clinically relevant doses, PpIX photobleaching throughout the skin was more profound following LED than LPDL and IPL exposure.

U2 - 10.1111/j.1600-0625.2012.01557.x

DO - 10.1111/j.1600-0625.2012.01557.x

M3 - Journal article

C2 - 22882358

SN - 1600-0625

VL - 21

SP - 745

EP - 750

JO - Experimental Dermatology

JF - Experimental Dermatology

IS - 10

ER -

Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources

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