Proteomic strategies in bladder cancer: From tissue to fluid and back

TY - JOUR

T1 - Proteomic strategies in bladder cancer

T2 - From tissue to fluid and back

AU - Gromov, Pavel

AU - Moreira, José M.A.

AU - Gromova, Irina

AU - Celis, Julio E.

PY - 2008/7/1

Y1 - 2008/7/1

N2 - We have applied protein expression profiling technologies in combination with immunohistochemistry, using fresh tissue and urine samples, to assess bladder cancer heterogeneity and prognosis as well as to generate protein markers for tumor progression and early diagnosis of the disease. Here, we review some selected lines of investigation and approaches undertaken by our laboratory, drawing on more than 15 years of experience in bladder cancer pboteomics, to high-light a number of issues that may be useful for researchers entering the field. In particular, we address the identification of markers for bladder cancer progression and exemplify the potential of gel-based proteomic profiling of urine samples for the early detection of urothelial carcinomas. In addition, we provide a brief description of a novel and highly promising source of biomarkers, the tumor interstitial fluid (TIF) that perfuses the tumor microenvironment.

AB - We have applied protein expression profiling technologies in combination with immunohistochemistry, using fresh tissue and urine samples, to assess bladder cancer heterogeneity and prognosis as well as to generate protein markers for tumor progression and early diagnosis of the disease. Here, we review some selected lines of investigation and approaches undertaken by our laboratory, drawing on more than 15 years of experience in bladder cancer pboteomics, to high-light a number of issues that may be useful for researchers entering the field. In particular, we address the identification of markers for bladder cancer progression and exemplify the potential of gel-based proteomic profiling of urine samples for the early detection of urothelial carcinomas. In addition, we provide a brief description of a novel and highly promising source of biomarkers, the tumor interstitial fluid (TIF) that perfuses the tumor microenvironment.

KW - Bladder cancer biomarkers

KW - Non-invasive diagnosis

UR - http://www.scopus.com/inward/record.url?scp=50849137854&partnerID=8YFLogxK

U2 - 10.1002/prca.200780163

DO - 10.1002/prca.200780163

M3 - Review

AN - SCOPUS:50849137854

SN - 1862-8346

VL - 2

SP - 974

EP - 988

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

IS - 7-8

ER -