Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network

Harriet Tempé Parsons; Georgia Drakakaki; Joshua L. Heazlewood

doi:10.3389/fpls.2012.00298

Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network

Harriet Tempé Parsons, Georgia Drakakaki, Joshua L. Heazlewood

Planteglycobiologi

18 Citationer (Scopus)

664 Downloads (Pure)

Abstract

The plant Golgi apparatus and trans-Golgi network are major endomembrane trafficking hubs within the plant cell and are involved in a diverse and vital series of functions to maintain plant growth and development. Recently, a series of disparate technical approaches have been used to isolate and characterize components of these complex organelles by mass spectrometry in the model plant Arabidopsis thaliana. Collectively, these studies have increased the number of Golgi and vesicular localized proteins identified by mass spectrometry to nearly 500 proteins. We have sought to provide a brief overview of these technical approaches and bring the datasets together to examine how they can reveal insights into the secretory pathway.

Originalsprog	Engelsk
Tidsskrift	Frontiers in Plant Science
Vol/bind	3
Antal sider	7
ISSN	1664-462X
DOI	https://doi.org/10.3389/fpls.2012.00298
Status	Udgivet - 3 jan. 2013

Emneord

Golgi
trans-Golgi network
proteomics
LOPIT
free-flow electrophoresis
Arabidopsis
SYP61

Adgang til dokumentet

10.3389/fpls.2012.00298

ProteomicdissectionoftheArabidopsisGolgiandtrans-GolginetworkForlagets udgivne version, 317 KB

Citationsformater

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KW - proteomics

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KW - free-flow electrophoresis

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