Proteomic approaches in circadian biology

Maria S Robles, Matthias Mann

12 Citationer (Scopus)

Abstract

Circadian clocks are endogenous oscillators that drive the rhythmic expression of a broad array of genes that orchestrate metabolism and physiology. Recent evidence indicates that posttranscriptional and posttranslational mechanisms play essential roles in modulating circadian gene expression, particularly for the molecular mechanism of the clock. In contrast to genetic technologies that have long been used to study circadian biology, proteomic approaches have so far been limited and, if applied at all, have used two-dimensional gel electrophoresis (2-DE). Here, we review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology. Large-scale, quantitative protein abundance measurements will help to understand to what extent the circadian clock drives system wide rhythms of protein abundance downstream of transcription regulation.
OriginalsprogEngelsk
TitelHandbook of experimental pharmacology : Circadian Clocks
Antal sider19
Publikationsdato2013
Sider389-407
DOI
StatusUdgivet - 2013
Udgivet eksterntJa
NavnHandbook of Experimental Pharmacology
ISSN0171-2004

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