Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection

Karina Juhl Rasmussen; Andreas Holm Mattsson; Katrine Pilely; Cecilie Antoinette Asferg; Oana Ciofu; Lars Vitved; Claus Koch; Michael Kemp

doi:10.1016/j.vaccine.2016.07.016

Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection

Karina Juhl Rasmussen^*, Andreas Holm Mattsson, Katrine Pilely, Cecilie Antoinette Asferg, Oana Ciofu, Lars Vitved, Claus Koch, Michael Kemp

^*Corresponding author af dette arbejde

3 Citationer (Scopus)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly growing problem, especially in hospitals where MRSA cause increased morbidity and mortality and a significant rise in health expenditures. As many strains of MRSA are resistant to other antimicrobials in addition to methicillin, there is an urgent need to institute non-antimicrobial measures, such as vaccination, against the spread of MRSA. With the aim of finding new protective antigens for vaccine development, this study used a proteome-wide in silico antigen prediction platform to screen the proteome of S. aureus strain MRSA252. Thirty-five different S. aureus proteins were identified, recombinantly expressed, and tested for protection in a lethal sepsis mouse model using S. aureus strain MRSA252 as the challenge organism. We found that 13 of the 35 recombinant peptides yielded significant protection and that 12 of these antigens were highly conserved across 70 completely sequenced S. aureus strains. Thus, this in silico platform was capable of identifying novel candidates for inclusion in future vaccines against MRSA.

Originalsprog	Engelsk
Tidsskrift	Vaccine
Vol/bind	34
Udgave nummer	38
Sider (fra-til)	4602-4609
Antal sider	8
ISSN	0264-410X
DOI	https://doi.org/10.1016/j.vaccine.2016.07.016
Status	Udgivet - 31 aug. 2016

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10.1016/j.vaccine.2016.07.016

Citationsformater

@article{4121683c5139419f99bc00f78729dbdb,

title = "Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection",

abstract = "Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly growing problem, especially in hospitals where MRSA cause increased morbidity and mortality and a significant rise in health expenditures. As many strains of MRSA are resistant to other antimicrobials in addition to methicillin, there is an urgent need to institute non-antimicrobial measures, such as vaccination, against the spread of MRSA. With the aim of finding new protective antigens for vaccine development, this study used a proteome-wide in silico antigen prediction platform to screen the proteome of S. aureus strain MRSA252. Thirty-five different S. aureus proteins were identified, recombinantly expressed, and tested for protection in a lethal sepsis mouse model using S. aureus strain MRSA252 as the challenge organism. We found that 13 of the 35 recombinant peptides yielded significant protection and that 12 of these antigens were highly conserved across 70 completely sequenced S. aureus strains. Thus, this in silico platform was capable of identifying novel candidates for inclusion in future vaccines against MRSA.",

keywords = "Antigen discovery, Proteome analysis, Staphylococcus aureus, Vaccine",

author = "Rasmussen, {Karina Juhl} and Mattsson, {Andreas Holm} and Katrine Pilely and Asferg, {Cecilie Antoinette} and Oana Ciofu and Lars Vitved and Claus Koch and Michael Kemp",

year = "2016",

month = aug,

day = "31",

doi = "10.1016/j.vaccine.2016.07.016",

language = "English",

volume = "34",

pages = "4602--4609",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier",

number = "38",

}

TY - JOUR

T1 - Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection

AU - Rasmussen, Karina Juhl

AU - Mattsson, Andreas Holm

AU - Pilely, Katrine

AU - Asferg, Cecilie Antoinette

AU - Ciofu, Oana

AU - Vitved, Lars

AU - Koch, Claus

AU - Kemp, Michael

PY - 2016/8/31

Y1 - 2016/8/31

N2 - Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly growing problem, especially in hospitals where MRSA cause increased morbidity and mortality and a significant rise in health expenditures. As many strains of MRSA are resistant to other antimicrobials in addition to methicillin, there is an urgent need to institute non-antimicrobial measures, such as vaccination, against the spread of MRSA. With the aim of finding new protective antigens for vaccine development, this study used a proteome-wide in silico antigen prediction platform to screen the proteome of S. aureus strain MRSA252. Thirty-five different S. aureus proteins were identified, recombinantly expressed, and tested for protection in a lethal sepsis mouse model using S. aureus strain MRSA252 as the challenge organism. We found that 13 of the 35 recombinant peptides yielded significant protection and that 12 of these antigens were highly conserved across 70 completely sequenced S. aureus strains. Thus, this in silico platform was capable of identifying novel candidates for inclusion in future vaccines against MRSA.

AB - Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly growing problem, especially in hospitals where MRSA cause increased morbidity and mortality and a significant rise in health expenditures. As many strains of MRSA are resistant to other antimicrobials in addition to methicillin, there is an urgent need to institute non-antimicrobial measures, such as vaccination, against the spread of MRSA. With the aim of finding new protective antigens for vaccine development, this study used a proteome-wide in silico antigen prediction platform to screen the proteome of S. aureus strain MRSA252. Thirty-five different S. aureus proteins were identified, recombinantly expressed, and tested for protection in a lethal sepsis mouse model using S. aureus strain MRSA252 as the challenge organism. We found that 13 of the 35 recombinant peptides yielded significant protection and that 12 of these antigens were highly conserved across 70 completely sequenced S. aureus strains. Thus, this in silico platform was capable of identifying novel candidates for inclusion in future vaccines against MRSA.

KW - Antigen discovery

KW - Proteome analysis

KW - Staphylococcus aureus

KW - Vaccine

U2 - 10.1016/j.vaccine.2016.07.016

DO - 10.1016/j.vaccine.2016.07.016

M3 - Journal article

C2 - 27496278

AN - SCOPUS:84996554088

SN - 0264-410X

VL - 34

SP - 4602

EP - 4609

JO - Vaccine

JF - Vaccine

IS - 38

ER -

Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection

Abstract

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