Proteoglycan Isolation and Analysis

Anne Woods; John R. Couchman

doi:10.1002/cpcb.59

Proteoglycan Isolation and Analysis

Abstract

Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

Originalsprog	Engelsk
Artikelnummer	e59
Tidsskrift	Current Protocols in Cell Biology
Vol/bind	80
Udgave nummer	1
Antal sider	21
ISSN	1934-2500
DOI	https://doi.org/10.1002/cpcb.59
Status	Udgivet - sep. 2018

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10.1002/cpcb.59

Citationsformater

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AB - Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

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Proteoglycan Isolation and Analysis

Abstract

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