Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function

TY - JOUR

T1 - Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function

AU - Tu, Fan

AU - Sedzinski, Jakub

AU - Ma, Yun

AU - Marcotte, Edward M

AU - Wallingford, John B

N1 - © 2018. Published by The Company of Biologists Ltd.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.

AB - Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.

U2 - 10.1242/jcs.206565

DO - 10.1242/jcs.206565

M3 - Journal article

C2 - 29180514

SN - 0021-9533

VL - 131

SP - 1

EP - 10

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 3

M1 - 206565

ER -