TY - JOUR
T1 - Profiling circulating miRNAs in serum from pigs infected with the porcine whipworm, Trichuris suis
AU - Hansen, Eline Palm
AU - Kringel, Helene
AU - Thamsborg, Stig Milan
AU - Jex, Aaron R.
AU - Nejsum, Peter
PY - 2016/6/15
Y1 - 2016/6/15
N2 - microRNAs (miRNAs) are recently discovered as key regulators of gene translation and are becoming increasingly recognized for their involvement in various diseases. This study investigates the miRNA profile in pig serum during the course of an infection with the gastrointestinal parasite, Trichuris suis. Of this panel, the expression of selected miRNAs in serum from T. suis infected and uninfected pigs were determined by quantitative real time PCR using Exiqon Human Panel assays at 0, 2, 4, 6, 8 and 10 weeks post first infection (wpi). One miRNA, ssc-let-7d-3p, was significantly up-regulated in infected pigs 8 wpi. Interestingly, ssc-let-7d-3p shows high complementary to tsu-let-7a, which is the most highly transcribed miRNA in T. suis. The let-7 family miRNAs have been shown to post-transcriptionally regulate the translation of the helminth-controlling cytokine, IL-13, in a murine model for asthma and we hypothesize possible interactions between these host- and parasite-derived miRNAs and their immunomodulating roles.
AB - microRNAs (miRNAs) are recently discovered as key regulators of gene translation and are becoming increasingly recognized for their involvement in various diseases. This study investigates the miRNA profile in pig serum during the course of an infection with the gastrointestinal parasite, Trichuris suis. Of this panel, the expression of selected miRNAs in serum from T. suis infected and uninfected pigs were determined by quantitative real time PCR using Exiqon Human Panel assays at 0, 2, 4, 6, 8 and 10 weeks post first infection (wpi). One miRNA, ssc-let-7d-3p, was significantly up-regulated in infected pigs 8 wpi. Interestingly, ssc-let-7d-3p shows high complementary to tsu-let-7a, which is the most highly transcribed miRNA in T. suis. The let-7 family miRNAs have been shown to post-transcriptionally regulate the translation of the helminth-controlling cytokine, IL-13, in a murine model for asthma and we hypothesize possible interactions between these host- and parasite-derived miRNAs and their immunomodulating roles.
KW - Faculty of Health and Medical Sciences
KW - Trichuris suis
KW - Whipworm
KW - Pig
KW - miRNA
KW - Biomarke
UR - https://www.sciencedirect.com/science/article/pii/S0304401717304727?via%3Dihub
U2 - 10.1016/j.vetpar.2016.03.025
DO - 10.1016/j.vetpar.2016.03.025
M3 - Journal article
C2 - 27198773
SN - 0304-4017
VL - 223
SP - 30
EP - 33
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -
