Production and crystallization of a panel of structure-based mutants of the human myelin peripheral membrane protein P2

TY - JOUR

T1 - Production and crystallization of a panel of structure-based mutants of the human myelin peripheral membrane protein P2

AU - Lehtimäki, Mari

AU - Laulumaa, Saara

AU - Ruskamo, Salla

AU - Kursula, Petri

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The myelin sheath is a multilayered membrane that surrounds and insulates axons in the nervous system. One of the proteins specific to the peripheral nerve myelin is P2, a protein that is able to stack lipid bilayers. With the goal of obtaining detailed information on the structure-function relationship of P2, 14 structure-based mutated variants of human P2 were generated and produced. The mutants were designed to potentially affect the binding of lipid bilayers by P2. All mutated variants were also crystallized and preliminary crystallographic data are presented. The structural data from the mutants will be combined with diverse functional assays in order to elucidate the fine details of P2 function at the molecular level.

AB - The myelin sheath is a multilayered membrane that surrounds and insulates axons in the nervous system. One of the proteins specific to the peripheral nerve myelin is P2, a protein that is able to stack lipid bilayers. With the goal of obtaining detailed information on the structure-function relationship of P2, 14 structure-based mutated variants of human P2 were generated and produced. The mutants were designed to potentially affect the binding of lipid bilayers by P2. All mutated variants were also crystallized and preliminary crystallographic data are presented. The structural data from the mutants will be combined with diverse functional assays in order to elucidate the fine details of P2 function at the molecular level.

KW - Amino Acid Substitution

KW - Crystallization

KW - Escherichia coli

KW - Humans

KW - Hydrophobic and Hydrophilic Interactions

KW - Models, Molecular

KW - Mutagenesis, Site-Directed

KW - Myelin P2 Protein/biosynthesis

KW - Protein Structure, Secondary

KW - Protein Structure, Tertiary

KW - X-Ray Diffraction

U2 - 10.1107/S1744309112039036

DO - 10.1107/S1744309112039036

M3 - Journal article

C2 - 23143249

SN - 2053-230X

VL - 68

SP - 1359

EP - 1362

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

IS - Pt 11

ER -