TY - JOUR
T1 - PRDM11 is dispensable for the maintenance and function of hematopoietic stem and progenitor cells
AU - Thoren, Lina A
AU - Fog, Cathrine K
AU - Jensen, Klaus T
AU - Buza-Vidas, Natalija
AU - Côme, Christophe
AU - Lund, Anders H.
AU - Porse, Bo T
N1 - © 2013.
PY - 2013/11
Y1 - 2013/11
N2 - Hematopoietic stem cells (HSC). 11Hematopoietic stem cell (HSC), hematopoietic stem and progenitor cell (HSPC), bone marrow (BM), bone marrow transplantation (BMT), acute myeloid leukemia (AML), peripheral blood (PB), multipotent progenitor (MPP), pre-megakaryocyte/erythroid (preMegE), megakaryocytic progenitor (MkP), pre-granulocyte/macrophage (preGM), granulocyte/macrophage progenitors (GMP), common lymphoid progenitors (CLP), colony forming unit erythroid (CFU-E), proErythroid (proE), colony forming unit megakaryocyte (CFU-Mk), colony forming unit granulocyte macrophage (CFU-GM), megakaryocyte (Mk), LSK (Lineage-, Sca1+, c-Kithi). supply organisms with life-long output of mature blood cells. To do so, the HSC pool size has to be maintained by HSC self-renewing divisions. PRDM3 and PRDM16 have been documented to regulate HSC self-renewal, maintenance and function. We found Prdm11 to have similar expression patterns in the hematopoietic stem and progenitor cell (HSPC) compartments as Prdm3 and Prdm16. Therefore, we undertook experiments to test if PRDM11 regulates HSC self-renewal, maintenance and function by investigating the Prdm11-/- mice. Our data shows that phenotypic HSPCs are intact in bone marrow (BM) of one-year-old Prdm11-/- mice. In addition, Prdm11-/- mice were able to fully regenerate the hematopoietic system upon BM transplantation (BMT) into lethally irradiated mice with a mild drop in lymphoid output only. Taken together, this suggests that PRDM11, in contrast to PRDM3 and PRDM16, is not directly involved in regulation of HSPCs in mice.
AB - Hematopoietic stem cells (HSC). 11Hematopoietic stem cell (HSC), hematopoietic stem and progenitor cell (HSPC), bone marrow (BM), bone marrow transplantation (BMT), acute myeloid leukemia (AML), peripheral blood (PB), multipotent progenitor (MPP), pre-megakaryocyte/erythroid (preMegE), megakaryocytic progenitor (MkP), pre-granulocyte/macrophage (preGM), granulocyte/macrophage progenitors (GMP), common lymphoid progenitors (CLP), colony forming unit erythroid (CFU-E), proErythroid (proE), colony forming unit megakaryocyte (CFU-Mk), colony forming unit granulocyte macrophage (CFU-GM), megakaryocyte (Mk), LSK (Lineage-, Sca1+, c-Kithi). supply organisms with life-long output of mature blood cells. To do so, the HSC pool size has to be maintained by HSC self-renewing divisions. PRDM3 and PRDM16 have been documented to regulate HSC self-renewal, maintenance and function. We found Prdm11 to have similar expression patterns in the hematopoietic stem and progenitor cell (HSPC) compartments as Prdm3 and Prdm16. Therefore, we undertook experiments to test if PRDM11 regulates HSC self-renewal, maintenance and function by investigating the Prdm11-/- mice. Our data shows that phenotypic HSPCs are intact in bone marrow (BM) of one-year-old Prdm11-/- mice. In addition, Prdm11-/- mice were able to fully regenerate the hematopoietic system upon BM transplantation (BMT) into lethally irradiated mice with a mild drop in lymphoid output only. Taken together, this suggests that PRDM11, in contrast to PRDM3 and PRDM16, is not directly involved in regulation of HSPCs in mice.
U2 - 10.1016/j.scr.2013.07.009
DO - 10.1016/j.scr.2013.07.009
M3 - Journal article
C2 - 23978475
SN - 1873-5061
VL - 11
SP - 1129
EP - 1136
JO - Stem Cell Research
JF - Stem Cell Research
IS - 3
ER -