PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica

TY - JOUR

T1 - PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica

AU - Navarre, William Wiley

AU - Zou, S Betty

AU - Roy, Hervé

AU - Xie, Jinglin Lucy

AU - Savchenko, Alexei

AU - Singer, Alexander

AU - Edvokimova, Elena

AU - Prost, Lynne R

AU - Kumar, Runjun

AU - Ibba, Michael

AU - Fang, Ferric C

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010/7/30

Y1 - 2010/7/30

N2 - We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-β-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.

AB - We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-β-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.

KW - Animals

KW - Bacterial Proteins

KW - Drug Resistance, Microbial

KW - Female

KW - Gene Expression Regulation, Bacterial

KW - Genomic Islands

KW - Lysine

KW - Lysine-tRNA Ligase

KW - Mice

KW - Peptide Elongation Factors

KW - Protein Processing, Post-Translational

KW - Salmonella enterica

KW - Virulence Factors

U2 - 10.1016/j.molcel.2010.06.021

DO - 10.1016/j.molcel.2010.06.021

M3 - Journal article

C2 - 20670890

SN - 1097-2765

VL - 39

SP - 209

EP - 221

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -