Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout, Oncorhynchus mykiss evaluated by a tail fin infection model.

TY - JOUR

T1 - Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout, Oncorhynchus mykiss evaluated by a tail fin infection model.

AU - Marana, Moonika Haahr

AU - Skov, Jakob

AU - Chettri, Jiwan Kumar

AU - Krossøy, Bjørn

AU - Dalsgaard, Inger

AU - Kania, Per Walter

AU - Buchmann, Kurt

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Rainbow trout, Oncorhynchus mykiss (Walbaum), are able to raise a protective immune response against Aeromonas salmonicida subsp. salmonicida (AS) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under Danish mariculture conditions. We elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. Rainbow trout juveniles were vaccinated by intraperitoneal (i.p.) injection with a bacterin of Aeromonas salmonicida subsp. salmonicida strain 090710-1/23 in combination with Vibrio anguillarum serotypes O1 and O2a supplemented with an oil adjuvant. Three concentrations of AS antigens were applied. Fish were subsequently challenged with the homologous bacterial strain administered by perforation of the tail fin epidermis and 60-s contact with live A. salmonicida bacteria. The infection method proved to be efficient and could differentiate efficacies of different vaccines. It was shown that protection and antibody production in exposed fish were positively correlated to the AS antigen concentration in the vaccine.

AB - Rainbow trout, Oncorhynchus mykiss (Walbaum), are able to raise a protective immune response against Aeromonas salmonicida subsp. salmonicida (AS) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under Danish mariculture conditions. We elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. Rainbow trout juveniles were vaccinated by intraperitoneal (i.p.) injection with a bacterin of Aeromonas salmonicida subsp. salmonicida strain 090710-1/23 in combination with Vibrio anguillarum serotypes O1 and O2a supplemented with an oil adjuvant. Three concentrations of AS antigens were applied. Fish were subsequently challenged with the homologous bacterial strain administered by perforation of the tail fin epidermis and 60-s contact with live A. salmonicida bacteria. The infection method proved to be efficient and could differentiate efficacies of different vaccines. It was shown that protection and antibody production in exposed fish were positively correlated to the AS antigen concentration in the vaccine.

U2 - 10.1111/jfd.12527

DO - 10.1111/jfd.12527

M3 - Journal article

C2 - 27593619

SN - 0140-7775

VL - 40

SP - 507

EP - 516

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

IS - 4

ER -