Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies

Susanna Cirera Salicio; Merete Fredholm

doi:10.1080/10495390801977584

Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies

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Abstract

The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.

Originalsprog	Engelsk
Tidsskrift	Animal Biotechnology (Print Edition)
Vol/bind	19
Udgave nummer	3
Sider (fra-til)	127-137
Antal sider	11
ISSN	1049-5398
DOI	https://doi.org/10.1080/10495390801977584
Status	Udgivet - 2008

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title = "Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies",

abstract = "The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.",

keywords = "Former LIFE faculty, Actinobacillus pleuropneumoniae, qPCR, SFTPB, SNP, Sus crofa",

author = "{Cirera Salicio}, Susanna and Merete Fredholm",

year = "2008",

doi = "10.1080/10495390801977584",

language = "English",

volume = "19",

pages = "127--137",

journal = "Animal Biotechnology",

issn = "1049-5398",

publisher = "Taylor & Francis",

number = "3",

}

TY - JOUR

T1 - Porcine lung surfactant protein B gene (SFTPB)

T2 - cDNA sequencing, chromosomal location, and expression studies

AU - Cirera Salicio, Susanna

AU - Fredholm, Merete

PY - 2008

Y1 - 2008

N2 - The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.

AB - The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.

KW - Former LIFE faculty

KW - Actinobacillus pleuropneumoniae

KW - qPCR

KW - SFTPB

KW - SNP

KW - Sus crofa

U2 - 10.1080/10495390801977584

DO - 10.1080/10495390801977584

M3 - Journal article

C2 - 18607785

SN - 1049-5398

VL - 19

SP - 127

EP - 137

JO - Animal Biotechnology

JF - Animal Biotechnology

IS - 3

ER -

Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies

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