Polymer Based Biosensor for Rapid Electrochemical Detection of Virus Infection of Human Cells

TY - JOUR

T1 - Polymer Based Biosensor for Rapid Electrochemical Detection of Virus Infection of Human Cells

AU - Kiilerich-Pedersen, Katrine

AU - Poulsen, Claus R.

AU - Jain, Titoo

AU - Rozlosnik, Noemi

PY - 2011/10/15

Y1 - 2011/10/15

N2 - The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy.Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3 h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.

AB - The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy.Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3 h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.

U2 - 10.1016/j.bios.2011.07.053

DO - 10.1016/j.bios.2011.07.053

M3 - Journal article

C2 - 21840702

SN - 0956-5663

VL - 28

SP - 386

EP - 392

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -