PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples

TY - JOUR

T1 - PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples

AU - Fazli, Mustafa

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

PY - 2014

Y1 - 2014

N2 - Fluorescence in situ hybridization with PNA probes (PNA-FISH) that target specific bacterial ribosomal RNA sequences is a powerful and rapid tool for identification of bacteria in clinical samples. PNA can diffuse readily through the bacterial cell wall due to its uncharged backbone, and PNA-FISH can be performed with high specificity due to the extraordinary thermal stability of RNA-PNA hybrid complexes. We describe a PNA-FISH procedure and provide examples of the application of PNA-FISH for the identification of bacteria in chronic wounds, cystic fibrosis lungs, and soft tissue fillers. In all these cases, bacteria can be identified in biofilm aggregates, which may explain their recalcitrance to antibiotic treatment.

AB - Fluorescence in situ hybridization with PNA probes (PNA-FISH) that target specific bacterial ribosomal RNA sequences is a powerful and rapid tool for identification of bacteria in clinical samples. PNA can diffuse readily through the bacterial cell wall due to its uncharged backbone, and PNA-FISH can be performed with high specificity due to the extraordinary thermal stability of RNA-PNA hybrid complexes. We describe a PNA-FISH procedure and provide examples of the application of PNA-FISH for the identification of bacteria in chronic wounds, cystic fibrosis lungs, and soft tissue fillers. In all these cases, bacteria can be identified in biofilm aggregates, which may explain their recalcitrance to antibiotic treatment.

U2 - 10.1007/978-1-4939-1459-3_21

DO - 10.1007/978-1-4939-1459-3_21

M3 - Journal article

C2 - 25218392

SN - 1064-3745

VL - 1211

SP - 261

EP - 271

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -