Pitfalls to avoid when using phage display for snake toxins

Andreas Hougaard Laustsen; Line Præst Lauridsen; Bruno Lomonte; Mikael Rørdam Andersen; Brian Lohse

doi:10.1016/j.toxicon.2016.12.010

Pitfalls to avoid when using phage display for snake toxins

Andreas Hougaard Laustsen, Line Præst Lauridsen, Bruno Lomonte, Mikael Rørdam Andersen, Brian Lohse

6 Citationer (Scopus)

Abstract

Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized production animals. Biotechnological innovations within envenoming therapies are thus warranted, and phage display technology may be a promising avenue for bringing antivenoms into the modern era of biologics. Although phage display technology represents a robust and high-throughput approach for the discovery of antibody-based antitoxins, several pitfalls may present themselves when animal toxins are used as targets for phage display selection. Here, we report selected critical challenges from our own phage display experiments associated with biotinylation of antigens, clone picking, and the presence of amber codons within antibody fragment structures in some phage display libraries. These challenges may be detrimental to the outcome of phage display experiments, and we aim to help other researchers avoiding these pitfalls by presenting their solutions.

Originalsprog	Engelsk
Tidsskrift	Toxicon
Vol/bind	126
Sider (fra-til)	79-89
Antal sider	11
ISSN	0041-0101
DOI	https://doi.org/10.1016/j.toxicon.2016.12.010
Status	Udgivet - 1 feb. 2017

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10.1016/j.toxicon.2016.12.010

Citationsformater

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title = "Pitfalls to avoid when using phage display for snake toxins",

abstract = "Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized production animals. Biotechnological innovations within envenoming therapies are thus warranted, and phage display technology may be a promising avenue for bringing antivenoms into the modern era of biologics. Although phage display technology represents a robust and high-throughput approach for the discovery of antibody-based antitoxins, several pitfalls may present themselves when animal toxins are used as targets for phage display selection. Here, we report selected critical challenges from our own phage display experiments associated with biotinylation of antigens, clone picking, and the presence of amber codons within antibody fragment structures in some phage display libraries. These challenges may be detrimental to the outcome of phage display experiments, and we aim to help other researchers avoiding these pitfalls by presenting their solutions.",

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T1 - Pitfalls to avoid when using phage display for snake toxins

AU - Laustsen, Andreas Hougaard

AU - Lauridsen, Line Præst

AU - Lomonte, Bruno

AU - Andersen, Mikael Rørdam

AU - Lohse, Brian

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized production animals. Biotechnological innovations within envenoming therapies are thus warranted, and phage display technology may be a promising avenue for bringing antivenoms into the modern era of biologics. Although phage display technology represents a robust and high-throughput approach for the discovery of antibody-based antitoxins, several pitfalls may present themselves when animal toxins are used as targets for phage display selection. Here, we report selected critical challenges from our own phage display experiments associated with biotinylation of antigens, clone picking, and the presence of amber codons within antibody fragment structures in some phage display libraries. These challenges may be detrimental to the outcome of phage display experiments, and we aim to help other researchers avoiding these pitfalls by presenting their solutions.

AB - Antivenoms against bites and stings from snakes, spiders, and scorpions are associated with immunological side effects and high cost of production, since these therapies are still derived from the serum of hyper-immunized production animals. Biotechnological innovations within envenoming therapies are thus warranted, and phage display technology may be a promising avenue for bringing antivenoms into the modern era of biologics. Although phage display technology represents a robust and high-throughput approach for the discovery of antibody-based antitoxins, several pitfalls may present themselves when animal toxins are used as targets for phage display selection. Here, we report selected critical challenges from our own phage display experiments associated with biotinylation of antigens, clone picking, and the presence of amber codons within antibody fragment structures in some phage display libraries. These challenges may be detrimental to the outcome of phage display experiments, and we aim to help other researchers avoiding these pitfalls by presenting their solutions.

U2 - 10.1016/j.toxicon.2016.12.010

DO - 10.1016/j.toxicon.2016.12.010

M3 - Journal article

C2 - 28017694

SN - 0041-0101

VL - 126

SP - 79

EP - 89

JO - Toxicon

JF - Toxicon

ER -

Pitfalls to avoid when using phage display for snake toxins

Abstract

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