Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer

TY - JOUR

T1 - Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer

AU - Liu, Ying

AU - Emilion, Gracy

AU - Mungall, Andrew J

AU - Dunham, Ian

AU - Beck, Stephan

AU - Le Meuth-Metzinger, Valerie G

AU - Shelling, Andrew N

AU - Charnock, Francis M L

AU - Ganesan, Trivadi S

PY - 2002/1/17

Y1 - 2002/1/17

N2 - We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.

AB - We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - COS Cells

KW - Chromosomes, Human, Pair 6

KW - Colony-Forming Units Assay

KW - Epithelial Cells

KW - Exons

KW - Expressed Sequence Tags

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Genes, Tumor Suppressor

KW - Genetic Markers

KW - Humans

KW - Introns

KW - Loss of Heterozygosity

KW - Molecular Sequence Data

KW - Ovarian Neoplasms

KW - Physical Chromosome Mapping

KW - RNA, Messenger

KW - Ribonucleases

KW - Sequence Alignment

KW - Transcription, Genetic

KW - Tumor Cells, Cultured

U2 - 10.1038/sj.onc.1205067

DO - 10.1038/sj.onc.1205067

M3 - Journal article

C2 - 11821951

SN - 0950-9232

VL - 21

SP - 387

EP - 399

JO - Oncogene

JF - Oncogene

IS - 3

ER -