Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

S. Svitashev; T. Bryngelsson; A. Vershinin; C. Pedersen; T. Säll; R. von Bothmer

doi:10.1007/BF00224500

Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

S. Svitashev^*, T. Bryngelsson, A. Vershinin, C. Pedersen, T. Säll, R. von Bothmer

^*Corresponding author af dette arbejde

Plante- og Jordvidenskab

75 Citationer (Scopus)

Abstract

A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. in situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.

Originalsprog	Engelsk
Tidsskrift	Theoretical and Applied Genetics
Vol/bind	89
Udgave nummer	7-8
Sider (fra-til)	801-810
Antal sider	10
ISSN	0040-5752
DOI	https://doi.org/10.1007/BF00224500
Status	Udgivet - 1 dec. 1994

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title = "Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences",

abstract = "A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. in situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.",

keywords = "Hordeum, In situ hybridization, Phylogeny, Repetitive DNA sequences, RFLP",

author = "S. Svitashev and T. Bryngelsson and A. Vershinin and C. Pedersen and T. S{\"a}ll and {von Bothmer}, R.",

year = "1994",

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T1 - Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

AU - Svitashev, S.

AU - Bryngelsson, T.

AU - Vershinin, A.

AU - Pedersen, C.

AU - Säll, T.

AU - von Bothmer, R.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. in situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.

AB - A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. in situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.

KW - Hordeum

KW - In situ hybridization

KW - Phylogeny

KW - Repetitive DNA sequences

KW - RFLP

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U2 - 10.1007/BF00224500

DO - 10.1007/BF00224500

M3 - Journal article

AN - SCOPUS:0028252884

SN - 0040-5752

VL - 89

SP - 801

EP - 810

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

IS - 7-8

ER -

Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

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