Phenotypical and genotypical characterisation of compact Kalanchoë lines produced by a non-GMO transformation strategy

TY - GEN

T1 - Phenotypical and genotypical characterisation of compact Kalanchoë lines produced by a non-GMO transformation strategy

AU - Lütken, Henrik Vlk

AU - Jensen, Erik Bjørn

AU - Wallström, Saba Victoria

AU - Müller, Renate

AU - Christensen, Brian

N1 - Conference code: 28

PY - 2012/11/15

Y1 - 2012/11/15

N2 - The aim of the present investigation was to evaluate a non-GMO transformation strategy to breed compact potted plants by using the naturally occurring bacterium Agrobacterium rhizogenes. Recently, it was shown that Kalanchoë blossfeldiana 'Molly' transformed with rol-genes (T1) exhibited a compact phenotype and improved ethylene tolerance. In the present study, the focus was the characterisation of the F1 and F2 progeny. F1 lines were produced by crossings between a selected T1 line and the commercial Kalanchoë cultivar 'Sarah'. F2 populations were produced by self-pollination of selected individual lines. A number of F1 and F2 lines were analysed genotypically. The presence of rol-genes was confirmed in both F1 and F2 plant lines exhibiting dwarfism. In the F1 progenies, the four rol-genes were linked and inherited dominantly in a Mendelian fashion. The segregation of the F2 population is currently being analysed. Phenotypical characterisation in a greenhouse trial revealed that both growth and development were affected. Several F1 and F2 plants exhibited a decreased plant height and changes in flowering and ethylene tolerance. Screening of F2 populations showed a different pattern of distribution of height and ethylene tolerance, depending on the parental lines. Populations derived from WT plants were taller than populations from parents containing rol-genes. The introduction of wild type genes from A. rhizogenes into ornamental plants is a valuable method of molecular breeding to obtain compact growth without using recombinant DNA technology. Compact potted plants can be obtained and lines without delayed flowering and improved ethylene tolerance are valuable for further breeding programmes.

AB - The aim of the present investigation was to evaluate a non-GMO transformation strategy to breed compact potted plants by using the naturally occurring bacterium Agrobacterium rhizogenes. Recently, it was shown that Kalanchoë blossfeldiana 'Molly' transformed with rol-genes (T1) exhibited a compact phenotype and improved ethylene tolerance. In the present study, the focus was the characterisation of the F1 and F2 progeny. F1 lines were produced by crossings between a selected T1 line and the commercial Kalanchoë cultivar 'Sarah'. F2 populations were produced by self-pollination of selected individual lines. A number of F1 and F2 lines were analysed genotypically. The presence of rol-genes was confirmed in both F1 and F2 plant lines exhibiting dwarfism. In the F1 progenies, the four rol-genes were linked and inherited dominantly in a Mendelian fashion. The segregation of the F2 population is currently being analysed. Phenotypical characterisation in a greenhouse trial revealed that both growth and development were affected. Several F1 and F2 plants exhibited a decreased plant height and changes in flowering and ethylene tolerance. Screening of F2 populations showed a different pattern of distribution of height and ethylene tolerance, depending on the parental lines. Populations derived from WT plants were taller than populations from parents containing rol-genes. The introduction of wild type genes from A. rhizogenes into ornamental plants is a valuable method of molecular breeding to obtain compact growth without using recombinant DNA technology. Compact potted plants can be obtained and lines without delayed flowering and improved ethylene tolerance are valuable for further breeding programmes.

M3 - Conference article

SN - 0567-7572

VL - 938

SP - 503

EP - 508

JO - Acta Horticulturae

JF - Acta Horticulturae

T2 - International Horticultural Congress on Science and Horticulture for People

Y2 - 22 August 2010 through 27 August 2010

ER -