TY - JOUR
T1 - Petrous bone diagenesis
T2 - a multi-analytical approach
AU - Kontopoulos, Ioannis
AU - Penkman, Kirsty
AU - McAllister, Graeme D.
AU - Lynnerup, Niels
AU - Damgaard, Peter B.
AU - Hansen, Henrik B.
AU - Allentoft, Morten E.
AU - Collins, Matthew J.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - The discovery of petrous bone as an excellent repository for ancient biomolecules has been a turning point in biomolecular archaeology, especially in aDNA research, but excessive and uncontrolled sampling could result in loss of this valuable resource for future research. This study reports on the histological (optical microscopy), physical (FTIR-ATR), elemental (CHN) and biochemical (collagen and DNA analysis) preservation of 15 human petrous bones spanning from the c. 2100 BCE to 1850 CE. Through the combined application of a number of diagenetic parameters (general histological index; infrared splitting factor; carbonate/phosphate ratio; amide/phosphate ratio; col wt%; % C, % N and C/N of whole bone and collagen; % endogenous DNA), we provide new insights into petrous bone micromorphological characteristics and diagenesis, and new evidence to enhance screening practices for aDNA and collagen analysis.
AB - The discovery of petrous bone as an excellent repository for ancient biomolecules has been a turning point in biomolecular archaeology, especially in aDNA research, but excessive and uncontrolled sampling could result in loss of this valuable resource for future research. This study reports on the histological (optical microscopy), physical (FTIR-ATR), elemental (CHN) and biochemical (collagen and DNA analysis) preservation of 15 human petrous bones spanning from the c. 2100 BCE to 1850 CE. Through the combined application of a number of diagenetic parameters (general histological index; infrared splitting factor; carbonate/phosphate ratio; amide/phosphate ratio; col wt%; % C, % N and C/N of whole bone and collagen; % endogenous DNA), we provide new insights into petrous bone micromorphological characteristics and diagenesis, and new evidence to enhance screening practices for aDNA and collagen analysis.
KW - aDNA
KW - Bioapatite
KW - Collagen
KW - Diagenesis
KW - Microstructure
KW - Petrous bone
UR - http://www.scopus.com/inward/record.url?scp=85060122834&partnerID=8YFLogxK
U2 - 10.1016/j.palaeo.2019.01.005
DO - 10.1016/j.palaeo.2019.01.005
M3 - Journal article
AN - SCOPUS:85060122834
SN - 0031-0182
VL - 518
SP - 143
EP - 154
JO - Palaeogeography, Palaeoclimatology, Palaeoecology - An International Journal for the Geo-Sciences
JF - Palaeogeography, Palaeoclimatology, Palaeoecology - An International Journal for the Geo-Sciences
ER -