Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne

Frido Welker; Mateja Hajdinjak; Sahra Talamo; Klervia Jaouen; Michael Dannemann; Francine David; Michèle Julien; Matthias Meyer; Janet Kelso; Ian Barnes; Selina Brace; Pepijn Kamminga; Roman Fischer; Benedikt M Kessler; John R Stewart; Svante Pääbo; Matthew J Collins; Jean-Jacques Hublin

doi:10.1073/pnas.1605834113

Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne

Frido Welker, Mateja Hajdinjak, Sahra Talamo, Klervia Jaouen, Michael Dannemann, Francine David, Michèle Julien, Matthias Meyer, Janet Kelso, Ian Barnes, Selina Brace, Pepijn Kamminga, Roman Fischer, Benedikt M Kessler, John R Stewart, Svante Pääbo, Matthew J Collins, Jean-Jacques Hublin

101 Citationer (Scopus)

Abstract

In Western Europe, the Middle to Upper Paleolithic transition is associated with the disappearance of Neandertals and the spread of anatomically modern humans (AMHs). Current chronological, behavioral, and biological models of this transitional period hinge on the Châtelperronian technocomplex. At the site of the Grotte du Renne, Arcy-sur-Cure, morphological Neandertal specimens are not directly dated but are contextually associated with the Châtelperronian, which contains bone points and beads. The association between Neandertals and this "transitional" assemblage has been controversial because of the lack either of a direct hominin radiocarbon date or of molecular confirmation of the Neandertal affiliation. Here we provide further evidence for a Neandertal-Châtelperronian association at the Grotte du Renne through biomolecular and chronological analysis. We identified 28 additional hominin specimens through zooarchaeology by mass spectrometry (ZooMS) screening of morphologically uninformative bone specimens from Châtelperronian layers at the Grotte du Renne. Next, we obtain an ancient hominin bone proteome through liquid chromatography-MS/MS analysis and error-tolerant amino acid sequence analysis. Analysis of this palaeoproteome allows us to provide phylogenetic and physiological information on these ancient hominin specimens. We distinguish Late Pleistocene clades within the genus Homo based on ancient protein evidence through the identification of an archaic-derived amino acid sequence for the collagen type X, alpha-1 (COL10α1) protein. We support this by obtaining ancient mtDNA sequences, which indicate a Neandertal ancestry for these specimens. Direct accelerator mass spectometry radiocarbon dating and Bayesian modeling confirm that the hominin specimens date to the Châtelperronian at the Grotte du Renne.

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Sciences of the United States of America
Vol/bind	113
Udgave nummer	40
Sider (fra-til)	11162-11167
Antal sider	6
ISSN	0027-8424
DOI	https://doi.org/10.1073/pnas.1605834113
Status	Udgivet - 4 okt. 2016

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10.1073/pnas.1605834113

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Welker, F., Hajdinjak, M., Talamo, S., Jaouen, K., Dannemann, M., David, F., Julien, M., Meyer, M., Kelso, J., Barnes, I., Brace, S., Kamminga, P., Fischer, R., Kessler, B. M., Stewart, J. R., Pääbo, S., Collins, M. J., & Hublin, J.-J. (2016). Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne. Proceedings of the National Academy of Sciences of the United States of America, 113(40), 11162-11167. https://doi.org/10.1073/pnas.1605834113

Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne. / Welker, Frido; Hajdinjak, Mateja; Talamo, Sahra et al.
I: Proceedings of the National Academy of Sciences of the United States of America, Bind 113, Nr. 40, 04.10.2016, s. 11162-11167.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Welker, F, Hajdinjak, M, Talamo, S, Jaouen, K, Dannemann, M, David, F, Julien, M, Meyer, M, Kelso, J, Barnes, I, Brace, S, Kamminga, P, Fischer, R, Kessler, BM, Stewart, JR, Pääbo, S, Collins, MJ & Hublin, J-J 2016, 'Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne', Proceedings of the National Academy of Sciences of the United States of America, bind 113, nr. 40, s. 11162-11167. https://doi.org/10.1073/pnas.1605834113

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title = "Palaeoproteomic evidence identifies archaic hominins associated with the Ch{\^a}telperronian at the Grotte du Renne",

abstract = "In Western Europe, the Middle to Upper Paleolithic transition is associated with the disappearance of Neandertals and the spread of anatomically modern humans (AMHs). Current chronological, behavioral, and biological models of this transitional period hinge on the Ch{\^a}telperronian technocomplex. At the site of the Grotte du Renne, Arcy-sur-Cure, morphological Neandertal specimens are not directly dated but are contextually associated with the Ch{\^a}telperronian, which contains bone points and beads. The association between Neandertals and this {"}transitional{"} assemblage has been controversial because of the lack either of a direct hominin radiocarbon date or of molecular confirmation of the Neandertal affiliation. Here we provide further evidence for a Neandertal-Ch{\^a}telperronian association at the Grotte du Renne through biomolecular and chronological analysis. We identified 28 additional hominin specimens through zooarchaeology by mass spectrometry (ZooMS) screening of morphologically uninformative bone specimens from Ch{\^a}telperronian layers at the Grotte du Renne. Next, we obtain an ancient hominin bone proteome through liquid chromatography-MS/MS analysis and error-tolerant amino acid sequence analysis. Analysis of this palaeoproteome allows us to provide phylogenetic and physiological information on these ancient hominin specimens. We distinguish Late Pleistocene clades within the genus Homo based on ancient protein evidence through the identification of an archaic-derived amino acid sequence for the collagen type X, alpha-1 (COL10α1) protein. We support this by obtaining ancient mtDNA sequences, which indicate a Neandertal ancestry for these specimens. Direct accelerator mass spectometry radiocarbon dating and Bayesian modeling confirm that the hominin specimens date to the Ch{\^a}telperronian at the Grotte du Renne.",

keywords = "Journal Article, Research Support, Non-U.S. Gov't",

author = "Frido Welker and Mateja Hajdinjak and Sahra Talamo and Klervia Jaouen and Michael Dannemann and Francine David and Mich{\`e}le Julien and Matthias Meyer and Janet Kelso and Ian Barnes and Selina Brace and Pepijn Kamminga and Roman Fischer and Kessler, {Benedikt M} and Stewart, {John R} and Svante P{\"a}{\"a}bo and Collins, {Matthew J} and Jean-Jacques Hublin",

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doi = "10.1073/pnas.1605834113",

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pages = "11162--11167",

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T1 - Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne

AU - Welker, Frido

AU - Hajdinjak, Mateja

AU - Talamo, Sahra

AU - Jaouen, Klervia

AU - Dannemann, Michael

AU - David, Francine

AU - Julien, Michèle

AU - Meyer, Matthias

AU - Kelso, Janet

AU - Barnes, Ian

AU - Brace, Selina

AU - Kamminga, Pepijn

AU - Fischer, Roman

AU - Kessler, Benedikt M

AU - Stewart, John R

AU - Pääbo, Svante

AU - Collins, Matthew J

AU - Hublin, Jean-Jacques

PY - 2016/10/4

Y1 - 2016/10/4

N2 - In Western Europe, the Middle to Upper Paleolithic transition is associated with the disappearance of Neandertals and the spread of anatomically modern humans (AMHs). Current chronological, behavioral, and biological models of this transitional period hinge on the Châtelperronian technocomplex. At the site of the Grotte du Renne, Arcy-sur-Cure, morphological Neandertal specimens are not directly dated but are contextually associated with the Châtelperronian, which contains bone points and beads. The association between Neandertals and this "transitional" assemblage has been controversial because of the lack either of a direct hominin radiocarbon date or of molecular confirmation of the Neandertal affiliation. Here we provide further evidence for a Neandertal-Châtelperronian association at the Grotte du Renne through biomolecular and chronological analysis. We identified 28 additional hominin specimens through zooarchaeology by mass spectrometry (ZooMS) screening of morphologically uninformative bone specimens from Châtelperronian layers at the Grotte du Renne. Next, we obtain an ancient hominin bone proteome through liquid chromatography-MS/MS analysis and error-tolerant amino acid sequence analysis. Analysis of this palaeoproteome allows us to provide phylogenetic and physiological information on these ancient hominin specimens. We distinguish Late Pleistocene clades within the genus Homo based on ancient protein evidence through the identification of an archaic-derived amino acid sequence for the collagen type X, alpha-1 (COL10α1) protein. We support this by obtaining ancient mtDNA sequences, which indicate a Neandertal ancestry for these specimens. Direct accelerator mass spectometry radiocarbon dating and Bayesian modeling confirm that the hominin specimens date to the Châtelperronian at the Grotte du Renne.

AB - In Western Europe, the Middle to Upper Paleolithic transition is associated with the disappearance of Neandertals and the spread of anatomically modern humans (AMHs). Current chronological, behavioral, and biological models of this transitional period hinge on the Châtelperronian technocomplex. At the site of the Grotte du Renne, Arcy-sur-Cure, morphological Neandertal specimens are not directly dated but are contextually associated with the Châtelperronian, which contains bone points and beads. The association between Neandertals and this "transitional" assemblage has been controversial because of the lack either of a direct hominin radiocarbon date or of molecular confirmation of the Neandertal affiliation. Here we provide further evidence for a Neandertal-Châtelperronian association at the Grotte du Renne through biomolecular and chronological analysis. We identified 28 additional hominin specimens through zooarchaeology by mass spectrometry (ZooMS) screening of morphologically uninformative bone specimens from Châtelperronian layers at the Grotte du Renne. Next, we obtain an ancient hominin bone proteome through liquid chromatography-MS/MS analysis and error-tolerant amino acid sequence analysis. Analysis of this palaeoproteome allows us to provide phylogenetic and physiological information on these ancient hominin specimens. We distinguish Late Pleistocene clades within the genus Homo based on ancient protein evidence through the identification of an archaic-derived amino acid sequence for the collagen type X, alpha-1 (COL10α1) protein. We support this by obtaining ancient mtDNA sequences, which indicate a Neandertal ancestry for these specimens. Direct accelerator mass spectometry radiocarbon dating and Bayesian modeling confirm that the hominin specimens date to the Châtelperronian at the Grotte du Renne.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1073/pnas.1605834113

DO - 10.1073/pnas.1605834113

M3 - Journal article

C2 - 27638212

SN - 0027-8424

VL - 113

SP - 11162

EP - 11167

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 40

ER -

Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne

Abstract

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