TY - JOUR
T1 - Osteoclasts secrete non-bone derived signals that induce bone formation
AU - Karsdal, Morten A
AU - Neutzsky-Wulff, Anita V
AU - Dziegiel, Morten Hanefeld
AU - Christiansen, Claus
AU - Henriksen, Kim
PY - 2008/2/8
Y1 - 2008/2/8
N2 - Bone turnover is a highly regulated process, where bone resorption in the normal healthy individual always is followed by bone formation in a manner referred to as coupling. Patients with osteopetrosis caused by defective acidification of the resorption lacuna have severely decreased resorption, in face of normal or even increased bone formation. This suggests that osteoclasts, not their resorptive activity, are important for sustaining bone formation. To investigate whether osteoclasts mediate control of bone formation by production of bone anabolic signals, we collected conditioned media (CM) from human osteoclasts cultured on either bone or plastic, and tested their effects on bone nodule formation by osteoblasts. Both types of CM were shown to dose-dependently induce bone nodule formation, whereas non-conditioned osteoclast culture medium had no effects. These data show that osteoclasts secrete non-bone derived factors, which induce preosteoblasts to form bone-like nodules, potentially explaining the imbalanced coupling seen in osteopetrotic patients.
AB - Bone turnover is a highly regulated process, where bone resorption in the normal healthy individual always is followed by bone formation in a manner referred to as coupling. Patients with osteopetrosis caused by defective acidification of the resorption lacuna have severely decreased resorption, in face of normal or even increased bone formation. This suggests that osteoclasts, not their resorptive activity, are important for sustaining bone formation. To investigate whether osteoclasts mediate control of bone formation by production of bone anabolic signals, we collected conditioned media (CM) from human osteoclasts cultured on either bone or plastic, and tested their effects on bone nodule formation by osteoblasts. Both types of CM were shown to dose-dependently induce bone nodule formation, whereas non-conditioned osteoclast culture medium had no effects. These data show that osteoclasts secrete non-bone derived factors, which induce preosteoblasts to form bone-like nodules, potentially explaining the imbalanced coupling seen in osteopetrotic patients.
KW - 3T3 Cells
KW - Animals
KW - Cell Communication
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Humans
KW - Mice
KW - Osteoclasts
KW - Osteogenesis
KW - Tissue Engineering
U2 - 10.1016/j.bbrc.2007.11.168
DO - 10.1016/j.bbrc.2007.11.168
M3 - Journal article
C2 - 18068671
SN - 0006-291X
VL - 366
SP - 483
EP - 488
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
