TY - JOUR
T1 - Osteoblastic response to pectin nanocoating on titanium surfaces
AU - Gurzawska, Katarzyna Aleksandra
AU - Svava, Rikke
AU - Yihua, Yu
AU - Haugshøj, Kenneth Brian
AU - Dirscherl, Kai
AU - Levery, Steven Bruce
AU - Chrestensen, Inge Byg
AU - Damager, Iben
AU - Weiss Nielsen, Martin
AU - Jørgensen, Bodil
AU - Jørgensen, Niklas Rye
AU - Gotfredsen, Klaus
PY - 2014
Y1 - 2014
N2 - Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.
AB - Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.
KW - Nanocoatings
KW - Osseointegration
KW - Osteoblasts
KW - Rhamnogalacturonan-I
KW - Surface properties
KW - Titanium
U2 - 10.1016/j.msec.2014.06.028
DO - 10.1016/j.msec.2014.06.028
M3 - Journal article
C2 - 25175196
AN - SCOPUS:84904720627
SN - 1873-0191
VL - 43
SP - 117
EP - 125
JO - Materials Science and Engineering C: Materials for Biological Applications
JF - Materials Science and Engineering C: Materials for Biological Applications
ER -