TY - JOUR
T1 - Orthologous and Paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria
AU - Rivera, Ivanna
AU - Molina, Rafael
AU - Lee, Mijoon
AU - Mobashery, Shahriar
AU - Hermoso, Juan A
PY - 2016/9
Y1 - 2016/9
N2 - Cell wall recycling and β-lactam antibiotic resistance are linked in Enterobacteriaceae and in Pseudomonas aeruginosa. This process involves a large number of murolytic enzymes, among them a cytoplasmic peptidoglycan amidase AmpD, which plays an essential role by cleaving the peptide stem from key intermediates en route to the β-lactamase production (a resistance mechanism) and cell wall recycling. Uniquely, P. aeruginosa has two additional paralogues of AmpD, designated AmpDh2 and AmpDh3, which are periplasmic enzymes. Despite the fact that AmpDh2 and AmpDh3 share a common motif for their respective catalytic domains, they are each comprised of multidomain architectures and exhibit distinct oligomerization properties. We review herein the structural and biochemical properties of orthologous and paralogous AmpD proteins and discuss their implication in cell wall recycling and antibiotic resistance processes.
AB - Cell wall recycling and β-lactam antibiotic resistance are linked in Enterobacteriaceae and in Pseudomonas aeruginosa. This process involves a large number of murolytic enzymes, among them a cytoplasmic peptidoglycan amidase AmpD, which plays an essential role by cleaving the peptide stem from key intermediates en route to the β-lactamase production (a resistance mechanism) and cell wall recycling. Uniquely, P. aeruginosa has two additional paralogues of AmpD, designated AmpDh2 and AmpDh3, which are periplasmic enzymes. Despite the fact that AmpDh2 and AmpDh3 share a common motif for their respective catalytic domains, they are each comprised of multidomain architectures and exhibit distinct oligomerization properties. We review herein the structural and biochemical properties of orthologous and paralogous AmpD proteins and discuss their implication in cell wall recycling and antibiotic resistance processes.
KW - Bacterial Proteins/chemistry
KW - Catalytic Domain
KW - Cell Wall/chemistry
KW - Drug Resistance, Microbial/genetics
KW - Enterobacteriaceae/enzymology
KW - Gene Expression
KW - Isoenzymes/chemistry
KW - Metalloproteases/chemistry
KW - Models, Molecular
KW - N-Acetylmuramoyl-L-alanine Amidase/chemistry
KW - Peptidoglycan/metabolism
KW - Periplasm/chemistry
KW - Protein Multimerization
KW - Protein Structure, Secondary
KW - Pseudomonas aeruginosa/enzymology
KW - Structural Homology, Protein
KW - Virulence Factors/chemistry
U2 - 10.1089/mdr.2016.0083
DO - 10.1089/mdr.2016.0083
M3 - Review
C2 - 27326855
SN - 1076-6294
VL - 22
SP - 470
EP - 476
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 6
ER -