Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium

Ulver Spangsberg Lorenzen, Gert H Hansen, E Michael Danielsen

9 Citationer (Scopus)

Abstract

Studies on bacterial enterotoxin-epithelium interactions require model systems capable of mimicking the events occurring at the molecular and cellular levels during intoxication. In this chapter, we describe organ culture as an often neglected alternative to whole-animal experiments or enterocyte-like cell lines. Like cell culture, organ culture is versatile and suitable for studying rapidly occurring events, such as enterotoxin binding and uptake. In addition, it is advantageous in offering an epithelium with more authentic permeability/barrier properties than any cell line, as well as a subepithelial lamina propria, harboring the immune cells of the gut mucosa.

OriginalsprogEngelsk
TitelSuperantigens : Methods and Protocols
RedaktørerAmanda J. Brosnahan
Antal sider8
Vol/bind1396
ForlagSpringer Science+Business Media
Publikationsdato2016
Sider159-166
Kapitel14
ISBN (Trykt)978-1-4939-3342-6
ISBN (Elektronisk)978-1-4939-3344-0
DOI
StatusUdgivet - 2016
NavnMethods in Molecular Biology
Vol/bind1396
ISSN1064-3745

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