Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces

Kasper Moth-Poulsen; Vesna Kofod-Hansen; Fadhil S. Kamounah; Nikos Hatzakis; Dimitrios Stamou; Kjeld Schaumburg; Jørn Bolstad Christensen

Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces

Kasper Moth-Poulsen, Vesna Kofod-Hansen, Fadhil S. Kamounah, Nikos Hatzakis, Dimitrios Stamou, Kjeld Schaumburg, Jørn Bolstad Christensen

Kemisk Institut

6 Citationer (Scopus)

Abstract

Attachment of molecules and proteins to surfaces is of great interest for the development of a large variety of applications. We present herein a novel approach to efficiently couple a molecule of choice to biological building blocks. We synthesized and employed a new derivative of 5-bromo-7-nitroindoline to attach nucleophilic molecules and proteins to gold surfaces by photochemical activation. The reaction can be seen as a photoactivated alternative to the activated ester type chemistries that are commonly used to attach proteins or molecules to surfaces. We characterize the reaction by UV-vis and NMR spectroscopy, and as test of principle experiment, we show that we can attach proteins to surfaces and demonstrate that we can functionalize gold nanoparticles by this optically induced cross-linking reaction.

Originalsprog	Engelsk
Tidsskrift	Bioconjugate Chemistry
Vol/bind	21
Sider (fra-til)	1056
Antal sider	1.061
ISSN	1043-1802
Status	Udgivet - 16 jun. 2010

Citationsformater

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title = "Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces",

abstract = "Attachment of molecules and proteins to surfaces is of great interest for the development of a large variety of applications. We present herein a novel approach to efficiently couple a molecule of choice to biological building blocks. We synthesized and employed a new derivative of 5-bromo-7-nitroindoline to attach nucleophilic molecules and proteins to gold surfaces by photochemical activation. The reaction can be seen as a photoactivated alternative to the activated ester type chemistries that are commonly used to attach proteins or molecules to surfaces. We characterize the reaction by UV-vis and NMR spectroscopy, and as test of principle experiment, we show that we can attach proteins to surfaces and demonstrate that we can functionalize gold nanoparticles by this optically induced cross-linking reaction.",

author = "Kasper Moth-Poulsen and Vesna Kofod-Hansen and {S. Kamounah}, Fadhil and Nikos Hatzakis and Dimitrios Stamou and Kjeld Schaumburg and Christensen, {J{\o}rn Bolstad}",

year = "2010",

month = jun,

day = "16",

language = "English",

volume = "21",

pages = "1056",

journal = "Bioconjugate Chemistry",

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TY - JOUR

T1 - Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces

AU - Moth-Poulsen, Kasper

AU - Kofod-Hansen, Vesna

AU - S. Kamounah, Fadhil

AU - Hatzakis, Nikos

AU - Stamou, Dimitrios

AU - Schaumburg, Kjeld

AU - Christensen, Jørn Bolstad

PY - 2010/6/16

Y1 - 2010/6/16

N2 - Attachment of molecules and proteins to surfaces is of great interest for the development of a large variety of applications. We present herein a novel approach to efficiently couple a molecule of choice to biological building blocks. We synthesized and employed a new derivative of 5-bromo-7-nitroindoline to attach nucleophilic molecules and proteins to gold surfaces by photochemical activation. The reaction can be seen as a photoactivated alternative to the activated ester type chemistries that are commonly used to attach proteins or molecules to surfaces. We characterize the reaction by UV-vis and NMR spectroscopy, and as test of principle experiment, we show that we can attach proteins to surfaces and demonstrate that we can functionalize gold nanoparticles by this optically induced cross-linking reaction.

AB - Attachment of molecules and proteins to surfaces is of great interest for the development of a large variety of applications. We present herein a novel approach to efficiently couple a molecule of choice to biological building blocks. We synthesized and employed a new derivative of 5-bromo-7-nitroindoline to attach nucleophilic molecules and proteins to gold surfaces by photochemical activation. The reaction can be seen as a photoactivated alternative to the activated ester type chemistries that are commonly used to attach proteins or molecules to surfaces. We characterize the reaction by UV-vis and NMR spectroscopy, and as test of principle experiment, we show that we can attach proteins to surfaces and demonstrate that we can functionalize gold nanoparticles by this optically induced cross-linking reaction.

M3 - Journal article

SN - 1043-1802

VL - 21

SP - 1056

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

ER -

Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces

Abstract

Fingeraftryk

Citationsformater