Optical manipulation of single molecules in the living cell

Kamilla Nørregaard; Liselotte Jauffred Pedersen; K. Berg-Sørensen; Lene Broeng Oddershede

doi:10.1039/c4cp00208c

Optical manipulation of single molecules in the living cell

Kamilla Nørregaard, Liselotte Jauffred Pedersen, K. Berg-Sørensen, Lene Broeng Oddershede

Biokompleksitet

28 Citationer (Scopus)

Abstract

Optical tweezers are the only nano-tools capable of manipulating and performing force-measurements on individual molecules and organelles within the living cell without performing destructive penetration through the cell wall and without the need for inserting a non-endogenous probe. Here, we describe how optical tweezers are used to manipulate individual molecules and perform accurate force and distance measurements within the complex cytoplasm of the living cell. Optical tweezers can grab individual molecules or organelles, if their optical contrast to the medium is large enough, as is the case, e.g., for lipid granules or chromosomes. However, often the molecule of interest is specifically attached to a handle manipulated by the optical trap. The most commonly used handles, their insertion into the cytoplasm, and the relevant micro-rheology of the cell are discussed here and we also review recent and exciting results achieved through optical force manipulation of individual molecules in vivo. This journal is

Originalsprog	Engelsk
Tidsskrift	Physical Chemistry Chemical Physics
Vol/bind	16
Udgave nummer	25
Sider (fra-til)	12614-12624
ISSN	1463-9076
DOI	https://doi.org/10.1039/c4cp00208c
Status	Udgivet - 7 jul. 2014

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10.1039/c4cp00208c

Citationsformater

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AU - Nørregaard, Kamilla

AU - Pedersen, Liselotte Jauffred

AU - Berg-Sørensen, K.

AU - Oddershede, Lene Broeng

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Optical manipulation of single molecules in the living cell

Abstract

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