Optical manipulation of hot nanoparticles can mediate selected cell fusion

TY - JOUR

T1 - Optical manipulation of hot nanoparticles can mediate selected cell fusion

AU - Oddershede, Lene Broeng

AU - Bahadori, Azra

AU - Bendix, Pól Martin

PY - 2017/4/18

Y1 - 2017/4/18

N2 - Metallic nanoparticles with diameters from 10 nm to 250 nm can be optically trapped and manipulated in 3D using a single tightly focused near infrared laser beam. This will result in a significant heating of the particle and its vicinity, with temperature increases easily reaching hundreds degrees Celsius. If such a hot metallic nanoparticle is brought into the contact zone between two cells or vesicles, this local temperature increase can cause a total fusion of the selected cells or vesicles. Upon fusion, both the membrane and the cargos become completely mixed and we also show that the cells remain viable after fusion. The presented method has potential for single-cell targeted drug delivery and for the creation of hybrid cells.

AB - Metallic nanoparticles with diameters from 10 nm to 250 nm can be optically trapped and manipulated in 3D using a single tightly focused near infrared laser beam. This will result in a significant heating of the particle and its vicinity, with temperature increases easily reaching hundreds degrees Celsius. If such a hot metallic nanoparticle is brought into the contact zone between two cells or vesicles, this local temperature increase can cause a total fusion of the selected cells or vesicles. Upon fusion, both the membrane and the cargos become completely mixed and we also show that the cells remain viable after fusion. The presented method has potential for single-cell targeted drug delivery and for the creation of hybrid cells.

U2 - 10.1117/12.2270424

DO - 10.1117/12.2270424

M3 - Journal article

SN - 0277-786X

VL - 10252

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 1025207

ER -