TY - JOUR
T1 - On the role of Cro in lambda prophage induction
AU - Svenningsen, Sine Lo
AU - Constantino, Nina
AU - Court, Donald L
AU - Adhya, Sankar
PY - 2005
Y1 - 2005
N2 - The lysogenic state of bacteriophage ¿ is exceptionally stable yet the prophage is readily induced in response to DNA damage. This delicate epigenetic switch is believed to be regulated by two proteins; the lysogenic maintenance promoting protein CI and the early lytic protein Cro. First, we confirm, in the native configuration, the previous observation that the DNA loop mediated by oligomerization of CI bound to two distinct operator regions (O L and O R), increases repression of the early lytic promoters and is important for stable maintenance of lysogeny. Second, we show that the presence of the cro gene might be unimportant for the lysogenic to lytic switch during induction of the ¿ prophage. We revisit the idea that Cro's primary role in induction is instead to mediate weak repression of the early lytic promoters.
AB - The lysogenic state of bacteriophage ¿ is exceptionally stable yet the prophage is readily induced in response to DNA damage. This delicate epigenetic switch is believed to be regulated by two proteins; the lysogenic maintenance promoting protein CI and the early lytic protein Cro. First, we confirm, in the native configuration, the previous observation that the DNA loop mediated by oligomerization of CI bound to two distinct operator regions (O L and O R), increases repression of the early lytic promoters and is important for stable maintenance of lysogeny. Second, we show that the presence of the cro gene might be unimportant for the lysogenic to lytic switch during induction of the ¿ prophage. We revisit the idea that Cro's primary role in induction is instead to mediate weak repression of the early lytic promoters.
U2 - 10.1073/pnas.0409839102
DO - 10.1073/pnas.0409839102
M3 - Journal article
C2 - 15728734
SN - 0027-8424
VL - 102
SP - 4465
EP - 4469
JO - Proceedings of the National Academy of Science of the United States of America
JF - Proceedings of the National Academy of Science of the United States of America
IS - 12
ER -