On the mechanism of oscillations in neutrophils

Jens Christian Brasen; Torben Barington; Lars Folke Olsen

doi:10.1016/j.bpc.2010.02.013

On the mechanism of oscillations in neutrophils

Jens Christian Brasen, Torben Barington, Lars Folke Olsen

Nyre- og Karforskning

6 Citationer (Scopus)

Abstract

We have investigated the regulation of the oscillatory generation of H₂O₂ and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H₂O₂ production are 180° out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H₂O₂ production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H₂O₂ production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.

Originalsprog	Engelsk
Tidsskrift	Biophysical Chemistry
Vol/bind	148
Udgave nummer	1-3
Sider (fra-til)	82-92
Antal sider	11
ISSN	0301-4622
DOI	https://doi.org/10.1016/j.bpc.2010.02.013
Status	Udgivet - 1 maj 2010

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10.1016/j.bpc.2010.02.013

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title = "On the mechanism of oscillations in neutrophils",

abstract = "We have investigated the regulation of the oscillatory generation of H2O2 and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H2O2 production are 180° out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H2O2 production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H2O2 production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.",

keywords = "1-Butanol, Calcium, Cell Count, Cytochalasin B, Feedback, Physiological, Humans, Hydrogen Peroxide, Models, Biological, NADP, Neutrophils, Phosphatidylinositol 3-Kinases, Phospholipase D, Protein Multimerization, Proto-Oncogene Proteins c-akt",

author = "Brasen, {Jens Christian} and Torben Barington and Olsen, {Lars Folke}",

year = "2010",

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doi = "10.1016/j.bpc.2010.02.013",

language = "English",

volume = "148",

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journal = "Biophysical Chemistry",

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TY - JOUR

T1 - On the mechanism of oscillations in neutrophils

AU - Brasen, Jens Christian

AU - Barington, Torben

AU - Olsen, Lars Folke

PY - 2010/5/1

Y1 - 2010/5/1

N2 - We have investigated the regulation of the oscillatory generation of H2O2 and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H2O2 production are 180° out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H2O2 production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H2O2 production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.

AB - We have investigated the regulation of the oscillatory generation of H2O2 and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H2O2 production are 180° out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H2O2 production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H2O2 production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.

KW - 1-Butanol

KW - Calcium

KW - Cell Count

KW - Cytochalasin B

KW - Feedback, Physiological

KW - Humans

KW - Hydrogen Peroxide

KW - Models, Biological

KW - NADP

KW - Neutrophils

KW - Phosphatidylinositol 3-Kinases

KW - Phospholipase D

KW - Protein Multimerization

KW - Proto-Oncogene Proteins c-akt

U2 - 10.1016/j.bpc.2010.02.013

DO - 10.1016/j.bpc.2010.02.013

M3 - Journal article

C2 - 20227815

SN - 0301-4622

VL - 148

SP - 82

EP - 92

JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 1-3

ER -

On the mechanism of oscillations in neutrophils

Abstract

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