Occurrence and identification of yeast species in fermented liquid feed for piglets

Klaus Gori; Marina Kryger Bjørklund; Nuria Canibe; Anni Øyan Pedersen; Lene Jespersen

doi:10.1007/s00248-010-9706-6

Occurrence and identification of yeast species in fermented liquid feed for piglets

Klaus Gori, Marina Kryger Bjørklund, Nuria Canibe, Anni Øyan Pedersen, Lene Jespersen

12 Citationer (Scopus)

Abstract

The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.

Originalsprog	Engelsk
Tidsskrift	Microbial Ecology
Vol/bind	61
Udgave nummer	1
Sider (fra-til)	146-153
Antal sider	8
ISSN	0095-3628
DOI	https://doi.org/10.1007/s00248-010-9706-6
Status	Udgivet - jan. 2011

Adgang til dokumentet

10.1007/s00248-010-9706-6

http://www.springerlink.com/content/82r12013843l0wu6/fulltext.pdf

Citationsformater

@article{4f7f3e41993e449583633a0b74a63c09,

title = "Occurrence and identification of yeast species in fermented liquid feed for piglets",

abstract = "The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.",

author = "Klaus Gori and Bj{\o}rklund, {Marina Kryger} and Nuria Canibe and Pedersen, {Anni {\O}yan} and Lene Jespersen",

year = "2011",

month = jan,

doi = "10.1007/s00248-010-9706-6",

language = "English",

volume = "61",

pages = "146--153",

journal = "Microbial Ecology",

issn = "0095-3628",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Occurrence and identification of yeast species in fermented liquid feed for piglets

AU - Gori, Klaus

AU - Bjørklund, Marina Kryger

AU - Canibe, Nuria

AU - Pedersen, Anni Øyan

AU - Jespersen, Lene

PY - 2011/1

Y1 - 2011/1

N2 - The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.

AB - The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.

U2 - 10.1007/s00248-010-9706-6

DO - 10.1007/s00248-010-9706-6

M3 - Journal article

C2 - 20574824

SN - 0095-3628

VL - 61

SP - 146

EP - 153

JO - Microbial Ecology

JF - Microbial Ecology

IS - 1

ER -