Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity

M D Kristensen; S M Petersen; K E Møller; M T Lund; M Hansen; C N Hansen; J Courraud; J W Helge; F Dela; C Prats

doi:10.1111/apha.13100

Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity

M D Kristensen, S M Petersen, K E Møller, M T Lund, M Hansen, C N Hansen, J Courraud, J W Helge, F Dela, C Prats

14 Citationer (Scopus)

Abstract

AIMS: Skeletal muscle lipid stores and mitochondrial function have been appointed as key players in obesity-induced insulin resistance. However, there are conflicting reports in the literature based on in vitro quantitative measurements. Here, we test the hypothesis that it is not the quantity but the quality that matters.

METHODS: This study combines quantitative and qualitative structural measurements of lipid stores and mitochondrial dynamics in skeletal muscle from lean subjects, and subjects with morbid obesity, with and without type 2 diabetes, before and after gastric bypass surgery.

RESULTS: The structural organization of muscle mitochondrial networks in type II muscle fibres from subjects with morbid obesity is impaired. In addition, the amount of skeletal muscle perilipin 2 protein per intramyocellular lipid is reduced in subjects with morbid obesity, resulting in qualitative alterations in perilipin 2 coat around some lipid droplets. Gastric bypass surgery-induced weight loss and insulin resistance remission were associated with decreases in intramyocellular lipid stores and, qualitative improvements in lipid droplets' morphology, perilipin 2 coat and mitochondrial dynamics.

CONCLUSION: Morbid obesity leads to severe qualitative alterations of both skeletal muscle lipid stores and mitochondrial networks. The degree of structural improvements after gastric bypass surgery was proportional to the improvements in whole body insulin sensitivity, suggesting an association between these events.

Originalsprog	Engelsk
Artikelnummer	e13100
Tidsskrift	Acta Physiologica (Print)
Vol/bind	224
Udgave nummer	4
Antal sider	15
ISSN	1748-1708
DOI	https://doi.org/10.1111/apha.13100
Status	Udgivet - dec. 2018

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10.1111/apha.13100

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Citationsformater

Kristensen, M. D., Petersen, S. M., Møller, K. E., Lund, M. T., Hansen, M., Hansen, C. N., Courraud, J., Helge, J. W., Dela, F., & Prats, C. (2018). Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity. Acta Physiologica (Print), 224(4), [e13100 ]. https://doi.org/10.1111/apha.13100

Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity. / Kristensen, M D; Petersen, S M; Møller, K E et al.

I: Acta Physiologica (Print), Bind 224, Nr. 4, e13100 , 12.2018.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Kristensen, MD, Petersen, SM, Møller, KE , Lund, MT, Hansen, M, Hansen, CN, Courraud, J, Helge, JW , Dela, F & Prats, C 2018, 'Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity', Acta Physiologica (Print), bind 224, nr. 4, e13100 . https://doi.org/10.1111/apha.13100

Kristensen MD, Petersen SM, Møller KE , Lund MT, Hansen M, Hansen CN et al. Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity. Acta Physiologica (Print). 2018 dec.;224(4):e13100 . doi: 10.1111/apha.13100

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abstract = "AIMS: Skeletal muscle lipid stores and mitochondrial function have been appointed as key players in obesity-induced insulin resistance. However, there are conflicting reports in the literature based on in vitro quantitative measurements. Here, we test the hypothesis that it is not the quantity but the quality that matters.METHODS: This study combines quantitative and qualitative structural measurements of lipid stores and mitochondrial dynamics in skeletal muscle from lean subjects, and subjects with morbid obesity, with and without type 2 diabetes, before and after gastric bypass surgery.RESULTS: The structural organization of muscle mitochondrial networks in type II muscle fibres from subjects with morbid obesity is impaired. In addition, the amount of skeletal muscle perilipin 2 protein per intramyocellular lipid is reduced in subjects with morbid obesity, resulting in qualitative alterations in perilipin 2 coat around some lipid droplets. Gastric bypass surgery-induced weight loss and insulin resistance remission were associated with decreases in intramyocellular lipid stores and, qualitative improvements in lipid droplets' morphology, perilipin 2 coat and mitochondrial dynamics.CONCLUSION: Morbid obesity leads to severe qualitative alterations of both skeletal muscle lipid stores and mitochondrial networks. The degree of structural improvements after gastric bypass surgery was proportional to the improvements in whole body insulin sensitivity, suggesting an association between these events.",

keywords = "diabetes, gastric bypass surgery, lipid droplets, metabolism, mitochondrial networks and skeletal muscle",

author = "Kristensen, {M D} and Petersen, {S M} and M{\o}ller, {K E} and Lund, {M T} and M Hansen and Hansen, {C N} and J Courraud and Helge, {J W} and F Dela and C Prats",

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T1 - Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity

AU - Kristensen, M D

AU - Petersen, S M

AU - Møller, K E

AU - Lund, M T

AU - Hansen, M

AU - Hansen, C N

AU - Courraud, J

AU - Helge, J W

AU - Dela, F

AU - Prats, C

PY - 2018/12

Y1 - 2018/12

N2 - AIMS: Skeletal muscle lipid stores and mitochondrial function have been appointed as key players in obesity-induced insulin resistance. However, there are conflicting reports in the literature based on in vitro quantitative measurements. Here, we test the hypothesis that it is not the quantity but the quality that matters.METHODS: This study combines quantitative and qualitative structural measurements of lipid stores and mitochondrial dynamics in skeletal muscle from lean subjects, and subjects with morbid obesity, with and without type 2 diabetes, before and after gastric bypass surgery.RESULTS: The structural organization of muscle mitochondrial networks in type II muscle fibres from subjects with morbid obesity is impaired. In addition, the amount of skeletal muscle perilipin 2 protein per intramyocellular lipid is reduced in subjects with morbid obesity, resulting in qualitative alterations in perilipin 2 coat around some lipid droplets. Gastric bypass surgery-induced weight loss and insulin resistance remission were associated with decreases in intramyocellular lipid stores and, qualitative improvements in lipid droplets' morphology, perilipin 2 coat and mitochondrial dynamics.CONCLUSION: Morbid obesity leads to severe qualitative alterations of both skeletal muscle lipid stores and mitochondrial networks. The degree of structural improvements after gastric bypass surgery was proportional to the improvements in whole body insulin sensitivity, suggesting an association between these events.

AB - AIMS: Skeletal muscle lipid stores and mitochondrial function have been appointed as key players in obesity-induced insulin resistance. However, there are conflicting reports in the literature based on in vitro quantitative measurements. Here, we test the hypothesis that it is not the quantity but the quality that matters.METHODS: This study combines quantitative and qualitative structural measurements of lipid stores and mitochondrial dynamics in skeletal muscle from lean subjects, and subjects with morbid obesity, with and without type 2 diabetes, before and after gastric bypass surgery.RESULTS: The structural organization of muscle mitochondrial networks in type II muscle fibres from subjects with morbid obesity is impaired. In addition, the amount of skeletal muscle perilipin 2 protein per intramyocellular lipid is reduced in subjects with morbid obesity, resulting in qualitative alterations in perilipin 2 coat around some lipid droplets. Gastric bypass surgery-induced weight loss and insulin resistance remission were associated with decreases in intramyocellular lipid stores and, qualitative improvements in lipid droplets' morphology, perilipin 2 coat and mitochondrial dynamics.CONCLUSION: Morbid obesity leads to severe qualitative alterations of both skeletal muscle lipid stores and mitochondrial networks. The degree of structural improvements after gastric bypass surgery was proportional to the improvements in whole body insulin sensitivity, suggesting an association between these events.

KW - diabetes

KW - gastric bypass surgery

KW - lipid droplets

KW - metabolism

KW - mitochondrial networks and skeletal muscle

U2 - 10.1111/apha.13100

DO - 10.1111/apha.13100

M3 - Journal article

C2 - 29791782

SN - 1748-1708

VL - 224

JO - Acta Physiologica

JF - Acta Physiologica

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