Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

Lívia B. Brenelli; Gabriela F. Persinoti; João Paulo L. Franco Cairo; Marcelo V. Liberato; Thiago Augusto Goncalves; Igor V. R. Otero; Pedro H. Mainardi; Claus Felby; Lara D. Sette; Fabio M. Squina

doi:10.1038/s41598-019-53608-1

Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

Lívia B. Brenelli, Gabriela F. Persinoti, João Paulo L. Franco Cairo, Marcelo V. Liberato, Thiago Augusto Goncalves, Igor V. R. Otero, Pedro H. Mainardi, Claus Felby, Lara D. Sette, Fabio M. Squina

LUKKET: Skov, natur og biomasse

9 Citationer (Scopus)

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Abstract

The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh_Lac1 and Pnh_Lac2, the first is the major secretory protein of the fungi. The Pnh_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications.

Originalsprog	Engelsk
Artikelnummer	17564
Tidsskrift	Scientific Reports
Vol/bind	9
Antal sider	15
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-019-53608-1
Status	Udgivet - 1 dec. 2019

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10.1038/s41598-019-53608-1Licens: CC BY

Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strainForlagets udgivne version, 2,23 MBLicens: CC BY

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Brenelli, L. B., Persinoti, G. F., Franco Cairo, J. P. L., Liberato, M. V., Goncalves, T. A., Otero, I. V. R., Mainardi, P. H., Felby, C., Sette, L. D., & Squina, F. M. (2019). Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. Scientific Reports, 9, [17564]. https://doi.org/10.1038/s41598-019-53608-1

Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. / Brenelli, Lívia B.; Persinoti, Gabriela F.; Franco Cairo, João Paulo L. et al.

I: Scientific Reports, Bind 9, 17564, 01.12.2019.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Brenelli, LB, Persinoti, GF, Franco Cairo, JPL, Liberato, MV, Goncalves, TA, Otero, IVR, Mainardi, PH, Felby, C, Sette, LD & Squina, FM 2019, 'Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain', Scientific Reports, bind 9, 17564. https://doi.org/10.1038/s41598-019-53608-1

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title = "Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain",

abstract = "The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh_Lac1 and Pnh_Lac2, the first is the major secretory protein of the fungi. The Pnh_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications.",

author = "Brenelli, {L{\'i}via B.} and Persinoti, {Gabriela F.} and {Franco Cairo}, {Jo{\~a}o Paulo L.} and Liberato, {Marcelo V.} and Goncalves, {Thiago Augusto} and Otero, {Igor V. R.} and Mainardi, {Pedro H.} and Claus Felby and Sette, {Lara D.} and Squina, {Fabio M.}",

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doi = "10.1038/s41598-019-53608-1",

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AU - Brenelli, Lívia B.

AU - Persinoti, Gabriela F.

AU - Franco Cairo, João Paulo L.

AU - Liberato, Marcelo V.

AU - Goncalves, Thiago Augusto

AU - Otero, Igor V. R.

AU - Mainardi, Pedro H.

AU - Felby, Claus

AU - Sette, Lara D.

AU - Squina, Fabio M.

PY - 2019/12/1

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N2 - The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh_Lac1 and Pnh_Lac2, the first is the major secretory protein of the fungi. The Pnh_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications.

AB - The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh_Lac1 and Pnh_Lac2, the first is the major secretory protein of the fungi. The Pnh_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications.

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DO - 10.1038/s41598-019-53608-1

M3 - Journal article

C2 - 31772294

SN - 2045-2322

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JO - Scientific Reports

JF - Scientific Reports

M1 - 17564

ER -

Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

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