Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system

Y Palarasah; C Nielsen; U Sprogøe; M L Christensen; S Lillevang; H O Madsen; A Bygum; C Koch; K Skjodt; M-O Skjoedt

doi:10.1111/j.1365-2249.2011.04322.x

Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system

Y Palarasah, C Nielsen, U Sprogøe, M L Christensen, S Lillevang, H O Madsen, A Bygum, C Koch, K Skjodt, M-O Skjoedt

19 Citationer (Scopus)

Abstract

Summary: Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.

Originalsprog	Engelsk
Tidsskrift	Clinical and Experimental Immunology
Vol/bind	164
Udgave nummer	3
Sider (fra-til)	388-95
Antal sider	8
ISSN	0009-9104
DOI	https://doi.org/10.1111/j.1365-2249.2011.04322.x
Status	Udgivet - jun. 2011

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10.1111/j.1365-2249.2011.04322.x

j.1365-2249.2011.04322.xForlagets udgivne version, 526 KB

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-2249.2011.04322.x

Citationsformater

Palarasah, Y., Nielsen, C., Sprogøe, U., Christensen, M. L., Lillevang, S., Madsen, H. O., Bygum, A., Koch, C., Skjodt, K., & Skjoedt, M.-O. (2011). Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system. Clinical and Experimental Immunology, 164(3), 388-95. https://doi.org/10.1111/j.1365-2249.2011.04322.x

Palarasah, Y, Nielsen, C, Sprogøe, U, Christensen, ML, Lillevang, S, Madsen, HO, Bygum, A, Koch, C, Skjodt, K & Skjoedt, M-O 2011, 'Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system', Clinical and Experimental Immunology, bind 164, nr. 3, s. 388-95. https://doi.org/10.1111/j.1365-2249.2011.04322.x

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abstract = "Summary: Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.",

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T1 - Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system

AU - Palarasah, Y

AU - Nielsen, C

AU - Sprogøe, U

AU - Christensen, M L

AU - Lillevang, S

AU - Madsen, H O

AU - Bygum, A

AU - Koch, C

AU - Skjodt, K

AU - Skjoedt, M-O

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Y1 - 2011/6

N2 - Summary: Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.

AB - Summary: Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.

KW - Adult

KW - Aged

KW - Complement Pathway, Alternative

KW - Complement Pathway, Classical

KW - Complement Pathway, Mannose-Binding Lectin

KW - Enzyme-Linked Immunosorbent Assay

KW - False Negative Reactions

KW - False Positive Reactions

KW - Female

KW - Humans

KW - Infection

KW - Kidney Diseases, Cystic

KW - Lupus Erythematosus, Systemic

KW - Male

KW - Middle Aged

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1365-2249.2011.04322.x

DO - 10.1111/j.1365-2249.2011.04322.x

M3 - Journal article

C2 - 21401574

SN - 0009-9104

VL - 164

SP - 388

EP - 395

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

IS - 3

ER -

Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system

Abstract

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